HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bernabucci, U. Articles by Nardone, A. Search for Related Content PubMed PubMed Citation Articles by Bernabucci, U. Articles by Nardone, A.

Markers of Oxidative Status in Plasma and Erythrocytes of Transition Dairy Cows During Hot Season¹

Department of Animal Production, University of Tuscia, via C. De Lellis, 01100 Viterbo, Italy

Corresponding author:
U. Bernabucci; e-mail:
bernab{at}unitus.it.

The study was carried out to assess whether hot seasons affect the oxidative status of transition dairy cows. Twenty Holstein cows were utilized. Eleven of those cows gave birth in spring; the remaining nine cows gave birth in summer. Oxidative status was evaluated from 21 d before to 35 d after calving, determining oxidative markers in plasma [glutathione peroxidase activity, thiol groups, reactive oxygen metabolites, and thiobarbituric acid reactive substances (TBARS)] and in erythrocytes [glutathione peroxidase activity (GSH-Px-E), intracellular thiols (SH), superoxide dismutase activity (SOD) and TBARS]. The season did not modify plasma oxidative markers. In contrast, cows exposed to moderate heat stress (39.5 ± 0.2°C rectal temperatures) due to summer temperature-humidity index (73.2 ± 2.5 mean daily THI), showed higher erythrocyte SOD, GSH-Px-E, SH, and TBARS compared with spring cows, indicating a condition of oxidative stress in summer transition dairy cows. Some changes (the increase of SOD, for example), represent compensatory changes the cow is undergoing in response to increased oxidative stress. Under the conditions compared, the effect of heat stress on oxidative status in transition dairy cows by use of plasma markers does not give enough information to reach definitive conclusions. Erythrocytes are an appropriate and sensitive model to study the oxidative status of transition dairy cows exposed to hot environments.

Abbreviation key: GSH-Px-E = erythrocyte glutathione peroxidase, GSH-Px-P = plasma glutathione peroxidase, MDA = malondialdehyde, PCV = packed cells volume, ROM = reactive oxygen metabolites, RR = respiration rates, RT = rectal temperatures, SH = intracellular thiols, SHp = plasma thiols, SOD = superoxide dismutase, SP = spring, SU = summer, TBARS = thiobarbituric acid reactive substances, THI = temperature-humidity index

Key Words: oxidative status marker • hot environment • transition dairy cow

This article has been cited by other articles:

				U. Bernabucci, L. Basirico, P. Morera, N. Lacetera, B. Ronchi, and A. Nardone Heat shock modulates adipokines expression in 3T3-L1 adipocytes J. Mol. Endocrinol., February 1, 2009; 42(2): 139 - 147. [Abstract] [Full Text] [PDF]

				M. Vazquez-Anon, J. Nocek, G. Bowman, T. Hampton, C. Atwell, P. Vazquez, and T. Jenkins Effects of Feeding a Dietary Antioxidant in Diets with Oxidized Fat on Lactation Performance and Antioxidant Status of the Cow J Dairy Sci, August 1, 2008; 91(8): 3165 - 3172. [Abstract] [Full Text] [PDF]

				R. J. Bouwstra, R. M. A. Goselink, P. Dobbelaar, M. Nielen, J. R. Newbold, and T. van Werven The Relationship Between Oxidative Damage and Vitamin E Concentration in Blood, Milk, and Liver Tissue from Vitamin E Supplemented and Nonsupplemented Periparturient Heifers J Dairy Sci, March 1, 2008; 91(3): 977 - 987. [Abstract] [Full Text] [PDF]

				N. C. Burke, G. Scaglia, K. E. Saker, D. J. Blodgett, and W. S. Swecker Jr Influence of endophyte consumption and heat stress on intravaginal temperatures, plasma lipid oxidation, blood selenium, and glutathione redox of mononuclear cells in heifers grazing tall fescue J Anim Sci, November 1, 2007; 85(11): 2932 - 2940. [Abstract] [Full Text] [PDF]

				M. Vazquez-Anon and T. Jenkins Effects of Feeding Oxidized Fat With or Without Dietary Antioxidants on Nutrient Digestibility, Microbial Nitrogen, and Fatty Acid Metabolism J Dairy Sci, September 1, 2007; 90(9): 4361 - 4367. [Abstract] [Full Text] [PDF]

				N. Lacetera, U. Bernabucci, D. Scalia, L. Basirico, P. Morera, and A. Nardone Heat Stress Elicits Different Responses in Peripheral Blood Mononuclear Cells from Brown Swiss and Holstein Cows J Dairy Sci, December 1, 2006; 89(12): 4606 - 4612. [Abstract] [Full Text] [PDF]

				U. Bernabucci, B. Ronchi, N. Lacetera, and A. Nardone Influence of Body Condition Score on Relationships Between Metabolic Status and Oxidative Stress in Periparturient Dairy Cows J Dairy Sci, June 1, 2005; 88(6): 2017 - 2026. [Abstract] [Full Text] [PDF]

				B. Lohrke, T. Viergutz, W. Kanitz, B. Losand, D. G. Weiss, and M. Simko Short Communication: Hydroperoxides in Circulating Lipids from Dairy Cows: Implications for Bioactivity of Endogenous-Oxidized Lipids J Dairy Sci, May 1, 2005; 88(5): 1708 - 1710. [Abstract] [Full Text] [PDF]

				M. Katsumata, M. Matsumoto, S. Kawakami, and Y. Kaji Effect of heat exposure on uncoupling protein-3 mRNA abundance in porcine skeletal muscle J Anim Sci, December 1, 2004; 82(12): 3493 - 3499. [Abstract] [Full Text] [PDF]