Bovine Somatotropin Attenuates Phorbol Ester-Induced Prostaglandin F₂ Production in Bovine Endometrial Cells

¹ Department of Animal Sciences, University of Florida, Gainesville 32611

The recent observation that bovine somatotropin (bST) treatment at a timed insemination improves pregnancy rates in lactating dairy cows raises the possibility that growth hormone (GH) may modulate the endocrine and biochemical cross talk between the conceptus and maternal uterus at the time of pregnancy establishment in cattle. The objective of this study was to characterize the cellular and molecular mechanisms by which exogenous GH affects phorbol esterinduced prostaglandin F₂ (PGF₂) production in cultured bovine endometrial (BEND) cells. Serum-deprived BEND cells were incubated with or without recombinant bovine GH (rbGH), insulin-like growth factor (IGF)-I, recombinant bovine interferon (rbIFN)- or a combination of rbGH + rbIFN- for 3 h and then treated with phorbol 12,13-dibutyrate (PDBu) for an additional 6 h. Exogenous PDBu increased PGF ₂ secretion and steady-state levels of COX-2 mRNA within 3 h. Priming of BEND cells with rbGH reduced PGF₂ response to PDBu, whereas cotreatment with IGF-I amplified PDBu induction of PGF₂ Preincubation of cell monolayers with rbIFN- suppressed PGF₂ and COX-2 mRNA responses to PDBu. Inhibitory effects of rbGH and rbIFN- on PDBu-induced PGF₂ production were additive. Results provide the first direct evidence that supplemental bST may interact with conceptus-secreted IFN- to modulate PGF₂ secretion at the critical time of maternal recognition of pregnancy.

Key Words: bovine somatotropin • endometrium • prostaglandin F₂ cattle

Submitted on August 13, 2001
Accepted on October 19, 2001

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