| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Animal Science and Animal Health, The Royal Veterinary and Agricultural University, Grønnegårdsvej 8, DK-1870 Frederiksberg C, Denmark
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, New York 14853, USA
Epivetko Aps, Tornager 2, DK-7600 Struer, Denmark
Corresponding author:
S. S. Nielsen; e-mail:
ssn{at}kvl.dk.
A longitudinal study was performed to determine the course of the milk antibody response in cows presumably infected with Mycobacterium avium subsp. paratuberculosis. Milk samples were collected repeatedly (1 to 10 times) from all lactating cows in seven Danish dairy herds. A total of 4289 observations from 812 cows was analyzed after exclusion of samples collected after 280 days in milk (DIM). The level of antibodies in the milk samples was assessed using an indirect ELISA.
A piece-wise linear random coefficient regression model was specified. The model controlled for the effect of herd, breed, laboratory effects, and age at first calving to estimate parity-specific antibody responses in relation to DIM. Separate antibody profiles were estimated for fecal culture-positive and fecal culture-negative cows. The resulting population average models showed higher antibody levels for fecal culture-positive cows and higher antibody levels with increasing parity. On average, the antibody response was high at the beginning and end of lactation. However, evaluating the cows individually indicated that most cows actually had quite stable ELISA levels throughout lactation, with some cows having higher levels than others. Thus, two criteria seem applicable to assess whether a cow is infected: stability and ELISA level. The random coefficients for each cow were highly significant. Thus, the study suggests that all cows can be classified into one of the four categories by combining the cow-level ELISA characteristics "stability" and "level" as an aid in the diagnosis of paratuberculosis and thereby substantially increasing the sensitivity of the ELISA.
Key Words: ELISA • paratuberculosis • immune dynamics
Abbreviation key: FC = fecal culture, OD = optical density
This article has been cited by other articles:
|
|
 |
|
  R. E. Click and C. L. Van Kampen Short communication: Progression of Johne's disease curtailed by a probiotic J Dairy Sci, October 1, 2009; 92(10): 4846 - 4851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Shin, D. Cho, and M. T. Collins Diagnosis of Bovine Paratuberculosis by a Novel Enzyme-Linked Immunosorbent Assay Based on Early Secreted Antigens of Mycobacterium avium subsp. paratuberculosis Clin. Vaccine Immunol., August 1, 2008; 15(8): 1277 - 1281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hinger, H. Brandt, S. Horner, and G. Erhardt Short Communication: Association Analysis of Microsatellites and Mycobacterium avium Subspecies paratuberculosis Antibody Response in German Holsteins J Dairy Sci, April 1, 2007; 90(4): 1957 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Lombard, T. M. Byrem, B. A. Wagner, and B. J. McCluskey Comparison of Milk and Serum Enzyme-linked Immunosorbent Assays for Diagnosis of Mycobacterium Avium Subspecies Paratuberculosis Infection in Dairy Cattle J Vet Diagn Invest, September 1, 2006; 18(5): 448 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Nielsen and N. Toft Age-Specific Characteristics of ELISA and Fecal Culture for Purpose-Specific Testing for Paratuberculosis J Dairy Sci, February 1, 2006; 89(2): 569 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Toft, S. S. Nielsen, and E. Jorgensen Continuous-Data Diagnostic Tests for Paratuberculosis as a Multistage Disease J Dairy Sci, November 1, 2005; 88(11): 3923 - 3931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. C. Klaas, C. Enevoldsen, A. K. Ersboll, and U. Tolle Cow-Related Risk Factors for Milk Leakage J Dairy Sci, January 1, 2005; 88(1): 128 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Mortensen, S. S. Nielsen, and P. Berg Genetic Variation and Heritability of the Antibody Response to Mycobacterium avium subspecies paratuberculosis in Danish Holstein Cows J Dairy Sci, July 1, 2004; 87(7): 2108 - 2113. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
