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1 Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches sur la Qualité et l'Hygiène des Aliments, 94704 Maisons-Alfort, France
2 UMR INRA-INAPG Physiologie de la Nutrition et Alimentation, 75231 Paris Cedex 05, France
Trans-octadecenoic acid composition of goat milk fat was studied by using silver-ion thin layer chromatography combined with gas-liquid chromatography. This analytical procedure also was used to investigate the effect of diet on trans-C18:1 fatty acid content in goat milk. Thirty-two goats were used in a 2 x 2 factorial arrangement and treatments. Two groups of goats received alfalfa hay at either a high or a low level of forage and two other groups received Rumiluz (dehydrated alfalfa from France Luzerne, Châlon en Champagne, France) at either a high or a low level of forage. Trans-C18:1 isomer proportions (relative to total fatty acids) were, respectively, 2.02% for the Rumiluz low-level group and 1.75% for the Rumiluz high-level group versus 1.71% for the alfalfa low-level group and 1.21% for the alfalfa high-level group. Goats fed on Rumiluz thus produced relatively higher levels of trans-C18:1 fatty acids than animals fed alfalfa hay. The results also showed that production of trans-C18:1 fatty acids increased when the level of forage in the diet decreased. Moreover, goat milk trans-C18:1 composition appeared similar to the cow milk profile. Vaccenic acid, trans-11-C18:1, was the major component and represented about 36.2% of total trans-C18:1 isomers.
Key Words: trans-C18:1 fatty acids • milk fat • diet • goat
Submitted on March 12, 2001
Accepted on September 27, 2001
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