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Journal of Dairy Science Vol. 84 No. 8 1783-1785
© 2001 by American Dairy Science Association ®
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Expression of clpX, an ATPase Subunit of the Clp Protease, Is Heat and Cold Shock Inducible in Lactococcus lactis

M. M. Skinner 1 and J. E. Trempy 1

1 Department of Microbiology, Oregon State University, Corvallis, Oregon 97331-3804

In this study, the clpX gene and surrounding sequences were cloned and sequenced from Lactococcus lactis. The putative clpX gene encodes a 411 amino acid polypeptide with a predicted molecular weight of 45.8 kDa. Analysis of the relative levels of clpX transcript revealed that in addition to a role in proteolysis of heat damaged proteins, ClpX may also be involved in cryo-protection.

Key Words: ClpX • ATPase • protease • heat shock • cold shock • Lactococcus lactis

Submitted on November 20, 2000
Accepted on April 2, 2001




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