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Journal of Dairy Science Vol. 84 No. 5 1034-1043
© 2001 by American Dairy Science Association ®
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Lipolysis of Butter Oil by Immobilized Lamb Pregastric Esterase: I. Uniresponse Kinetics—pH and Temperature Effects

C. G. Hill Jr. 1, S. Ghannouchi 1, and H. S. Garcia 2

1 Department of Chemical Engineering University of Wisconsin-Madison 1415 Engineering Drive, Madison, WI 53706
2 UNIDA-Instituto Tecnológico de Veracruz Apdo. Postal 1420, Veracruz, Ver., México

Lamb pregastric esterase, immobilized by physical adsorption on microporous polypropylene in a hollow fiber reactor, has been employed to effect the continuous hydrolysis of the triglycerides in butter oil. Experimental data were obtained at temperatures from 35 to 45°C and pH values from 5.5 to 6.5. The overall rate of hydrolysis was fastest at 40°C and a pH of 6.0. Nonlinear regression methods were employed to determine the kinetic parameters of rate expressions based on a generic Ping-Pong Bi Bi mechanism. The best nonlinear fit of the data was consistent with a mechanism that assumes that acylation of the enzyme is the rate-limiting step in the hydrolysis reaction.

Key Words: lamb pregastric esterase • lipolyzed butter oil • hollow fiber reactor • immobilized lipase

Submitted on September 5, 2000
Accepted on December 19, 2000






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Copyright © 2001 by the American Dairy Science Association ®.