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1 Istituto di Microbiologia, Facoltá di Agraria, U.C.S.C., Via Emilia Parmense 84, I-29100, Piacenza, Italy
2 VTT Biotechnology and Food Research, P.O. Box 1501, FIN-02044 VTT, Finland
3 Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland
4 Harjula Hospital, P.O. Box 38, FIN-70101, Kuopio, Finland
A wild-type Lactobacillus crispatus, showing a cell aggregation phenotype and its spontaneous nonaggregating mutant were compared for their in vitro adhesion properties to human ileal mucus and to a cultured human colonic cell line (Caco2) and for their in vivo colonization and adhesion potential with colonoscopy patients as volunteers in feeding trials. The wild-type strain adhered better to mucus or to Caco2 cells than did the mutant. Altogether, three human trials with the wild type and two with the mutant strain were performed. In two of the trials, the wild type could be recovered from either fecal samples or biopsies taken from the colon, while the mutant strain could not be demonstrated in either of the trials where it was used. The L. crispatus colonies recovered from the trials were often mixed, and several enterococci and lactobacillus strains coaggregating with L. crispatus wild type could be isolated. The results indicate that the surface-mediated properties, such as aggregation, of lactobacilli can have a role in adhesion and colonization.
Key Words: probiotics • aggregation • colonization • Lactobacillus
Submitted on August 1, 2000
Accepted on December 15, 2000
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