JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 84 No. 12 2641-2648
© 2001 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Piccinini, R.
Right arrow Articles by Zecconi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Piccinini, R.
Right arrow Articles by Zecconi, A.

Relationship Among Plasmids Recovered from Staphylococcus aureus, Milk Leukocytes, and Antimicrobial Resistance

R. Piccinini 1 and A. Zecconi 1

1 Università degli Studi di Milano, Dip Patologia Animale, Igiene e Sanità Pubblica Veterinaria-Sezione di Malattie Infettive, Via Celoria 10, 20133 Milano, Italy

The efficacy and efficiency of mastitis control schemes in reducing the incidence of Staphylococcus aureus mastitis could be improved if the different strains of Staph. aureus were characterized by their pathogenicity. Plasmid finger printing of Staph. aureus strains could be a rather simple and efficient method to characterize the strains and their interaction with host cellular immune defenses. The aim of this study was to investigate the presence and distribution of plasmids in strains of Staph. aureus isolated from bovine mastitis and their relationship with mammary gland immune defenses and antimicrobial resistance. Overall, nine different plasmids were identified by their molecular weight. One or more plasmids were isolated from 293 strains (96.4%), and only 11 strains did not show the presence of any plasmid. A significant difference in polymorphonuclear neutrophil proportions was observed in association with 23-kb, 9416-bp, 2027-bp, or 1353-bp plasmids, and for macrophage proportions, significant differences were observed for 9416-bp, 6557-bp, or 1353-bp plasmids. Penicillin G minimum inhibitory concentration values showed significant differences when 6557-bp, 4361-bp, 2322-bp, or 2027-bp plasmids were present, and weak or no differences were observed for cephacetrile, rifamycin, and norfloxacin. The presence of 2027-bp plasmid was associated with a reduced neutrophil response and an increased resistance against ß-lactam antimicrobials; therefore, the strains carrying this plasmid have a higher probability to invade and colonize mammary gland, to survive to antimicrobial therapy, and to remain undetected. Results confirmed that plasmid fingerprinting is a simple and effective way to characterize Staph. aureus strains.

Key Words: Staphylococcus aureus • plasmids • anti-microbial resistance • leukocytes

Submitted on January 18, 2001
Accepted on July 10, 2001




This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
J. E. Olsen, H. Christensen, and F. M. Aarestrup
Diversity and evolution of blaZ from Staphylococcus aureus and coagulase-negative staphylococci
J. Antimicrob. Chemother., March 1, 2006; 57(3): 450 - 460.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
E. M. Smith, L. E. Green, G. F. Medley, H. E. Bird, L. K. Fox, Y. H. Schukken, J. V. Kruze, A. J. Bradley, R. N. Zadoks, and C. G. Dowson
Multilocus Sequence Typing of Intercontinental Bovine Staphylococcus aureus Isolates
J. Clin. Microbiol., September 1, 2005; 43(9): 4737 - 4743.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Dairy Science Association ®.