JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 83 No. 9 2049-2056
© 2000 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berthiaume, R.
Right arrow Articles by McBride, B. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berthiaume, R.
Right arrow Articles by McBride, B. W.

Comparison of the In Situ and In Vivo Intestinal Disappearance of Ruminally Protected Methionine

R. Berthiaume 1, H. Lapierre 2, M. Stevenson 3, N. Coté 1, and B. W. McBride

1 University of Guelph, Guelph, On, Canada NIG 2W1
2 Agriculture and Agri-Food Canada, Lennoxville, Qc, Canada J1M 1Z3
3 Degussa Canada ltd., Burlington, On, Canada L7R 3Y8

We designed a switchback expriment to compare the intestinal disappearance of ruminally protected Met determined in situ or in vivo over three consecutive periods. Four nonlactating Holstein heifers (477 ± 36 kg) with cannulae in the rumen, duodenum, and ileum were fed a diet based on timothy silage to meet requirements for maintenance (dry matter intake = 8.72 ± 0.15 kg). A total of 16 bags, containing 1.5 g of ruminally protected Met, were incubated in the rumen (4.5 h) of each cow and transferred to an acid-pepsin solution to simulate the abomasum (2.5 h). Following each incubation, bags were recovered and three bags were dried at 55°C and analyzed for Met. Remaining bags were introduced directly into the duodenal or ileal cannula for the in situ method, while for the in vivo method, the content of remaining bags was transferred into gelatin capsules before their introduction in the duodenal or ileal cannula. Spot samples of digesta were collected during a 96-h period, with Co-EDTA and Cr-mordanted fiber used as indigestible markers to estimate in vivo digestibility. The disappearance of Met in the small intestine determined in vivo tended to be higher than in situ (74.45 vs. 43.65 ± 1.79%). Our results indicate that when used to assess intestinal availability of ruminally protected Met, the mobile nylon bag technique can underestimate the true bioavailability of Met.

Key Words: methionine • ruminally protected amino acids • dairy heifers • intestinal digestibility

Submitted on November 24, 1999
Accepted on March 17, 2000




This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
S. I. Borucki Castro, L. E. Phillip, H. Lapierre, P. W. Jardon, and R. Berthiaume
Ruminal Degradability and Intestinal Digestibility of Protein and Amino Acids in Treated Soybean Meal Products
J Dairy Sci, February 1, 2007; 90(2): 810 - 822.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
C. Leonardi, M. Stevenson, and L. E. Armentano
Effect of Two Levels of Crude Protein and Methionine Supplementation on Performance of Dairy Cows
J Dairy Sci, December 1, 2003; 86(12): 4033 - 4042.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Dairy Science Association ®.