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1 Nutrient Conservation and Metabolism Laboratory, USDA-ARS, Building 200, BARC East, Beltsville, MD 20705
The objective of this study was to investigate the feasibility of using near-infrared reflectance spectroscopy (NIRS) to determine nutrient concentrations in dairy manures. We assayed diverse dairy manures (n = 107) collected from dairy farms in the northeastern United States (CT, MD, NY, PA, and VA) by conventional means and NIRS for total C, total N, NH3-N, moisture, P, K, and pH. Samples were scanned from 400 to 2498 nm in polyethylene bags on a FOSS-NIR-Systems Model 6500 scanning monochromator equipped with a sample transport device. We developed calibrations using a one-out cross validation procedure under partial least-squares regression. Preliminary results showed that eight samples were outliers either because of inaccurate conventional analysis or because they were uncharacteristic (i.e., two samples had moisture content below 72%, while all others were above 78%). These outliers were removed from further consideration. Final calibration results with the remaining 99 samples demonstrated that NIRS can accurately determine the moisture (r2 = 0.945, root mean squared deviation or RMSD = 1.0%), total C (r2 = 0.950, RMSD = 0.40%), total N (r2 = 0.956, RMSD = 0.030%), and NH3-N (r2 = 0.967, RMSD = 0.013%) concentrations, but not P or K concentrations in dairy manures. In conclusion, NIRS was shown to be a viable alternative to conventional analysis procedures for determining moisture, total C, total N, and NH3-N in a very diverse set of dairy manures.
Key Words: near-infrared • manure • carbon • nitrogen
Submitted on October 14, 1999
Accepted on March 2, 2000
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