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Journal of Dairy Science Vol. 83 No. 7 1448-1450
© 2000 by American Dairy Science Association ®
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Short Communication: Salt Extends the Upper Temperature Limit for Growth of Lactococcus lactis ssp. cremoris on Solid M17 Medium

M. Kilstrup and K. Hammer 1

1 Department of Microbiology, Technical University of Denmark, DK2800 Lyngby, Denmark

We have determined conditions for plating of the Lactococcus lactis ssp. cremoris laboratory strain MG1363 on solid M17 broth at 38°C, which is required for the optimal use of the pGhost plasmids. The addition of 1% NaCl (or KCl, potassium acetate, or sucrose at 170 mM) to M17 agar plates results in extension of the upper temperature limit for growth from 37 to 40°C; no decrease in plating efficiency was detected from 30 to 39°C.

Key Words: salt • Lactococcus lactis ssp. cremoris • M17 medium

Submitted on December 8, 1999
Accepted on February 10, 2000




This article has been cited by other articles:


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J. Haaber, S. Moineau, L.-C. Fortier, and K. Hammer
AbiV, a Novel Antiphage Abortive Infection Mechanism on the Chromosome of Lactococcus lactis subsp. cremoris MG1363
Appl. Envir. Microbiol., November 1, 2008; 74(21): 6528 - 6537.
[Abstract] [Full Text] [PDF]


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