JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 83 No. 7 1434-1440
© 2000 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saito, T.
Right arrow Articles by Itoh, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saito, T.
Right arrow Articles by Itoh, T.

Isolation and Structural Analysis of Antihypertensive Peptides That Exist Naturally in Gouda Cheese

T. Saito 1, T. Nakamura 1, H. Kitazawa 1, Y. Kawai 1, and T. Itoh 1

1 Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan

Seven kinds of ripened cheeses (8-mo-aged and 24-mo-aged Gouda, Emmental, Blue, Camembert, Edam, and Havarti) were homogenized with distilled water, and water-soluble peptides were prepared by C-18 hydrophobic chromatography. The inhibitory activity to angiotensin I-converting enzyme and decrease in the systolic blood pressure in spontaneously hypertensive rats were measured before and after oral administration of each peptide sample. The strongest depressive effect in the systolic blood pressure –24.7 mm Hg) and intensive inhibitory activity to angiotensin I-converting enzyme (75.7%) were detected in the peptides from 8-mo-aged Gouda cheese. Four peptides were isolated by HPLC with reverse-phase and gel filtration modes. Their chemical structures and origins, clarified by combination analyses of protein sequencing, amino acid composition, and mass spectrometry, were as follows: peptide A, Arg-Pro-Lys-His-Pro-Ile-Lys-His-Gln [alphas1-casein (CN), B-8P; f 1-9]; peptide B, Arg-Pro-Lys-His-Pro-Ile-Lys-His-Gln-Gly-Leu-Pro-Gln (alphas1-CN, B-8P; f 1-13); peptide F, Tyr-Pro-Phe-Pro-Gly-Pro-Ile-Pro-Asn (ß-CN, A2-5P; f 60-68); and peptide G, Met-Pro-Phe-Pro-Lys-Tyr-Pro-Val-Gln-Pro-Phe (ß-CN, A2-5P; f 109-119). Peptides A and F, which were chemically synthesized, showed potent angiotensin I-converting enzyme inhibitory activity with little antihypertensive effects.

Key Words: Gouda cheese • peptides • angiotensin-converting enzyme • antihypertensivity

Submitted on October 3, 1999
Accepted on January 24, 2000




This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
T. K. Dalsgaard, C. W. Heegaard, and L. B. Larsen
Plasmin Digestion of Photooxidized Milk Proteins
J Dairy Sci, June 1, 2008; 91(6): 2175 - 2183.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
U. Butikofer, J. Meyer, R. Sieber, B. Walther, and D. Wechsler
Occurrence of the Angiotensin-Converting Enzyme-Inhibiting Tripeptides Val-Pro-Pro and Ile-Pro-Pro in Different Cheese Varieties of Swiss Origin
J Dairy Sci, January 1, 2008; 91(1): 29 - 38.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
W. Qi, R. X. Su, Z. M. He, Y. B. Zhang, and F. M. Jin
Pepsin-Induced Changes in the Size and Molecular Weight Distribution of Bovine Casein During Enzymatic Hydrolysis
J Dairy Sci, November 1, 2007; 90(11): 5004 - 5011.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
S. V. Silva, A. Pihlanto, and F. X. Malcata
Bioactive Peptides in Ovine and Caprine Cheeselike Systems Prepared with Proteases from Cynara cardunculus.
J Dairy Sci, September 1, 2006; 89(9): 3336 - 3344.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. Miguel and A. Aleixandre
Antihypertensive Peptides Derived from Egg Proteins
J. Nutr., June 1, 2006; 136(6): 1457 - 1460.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
A. Quiros, B. Hernandez-Ledesma, M. Ramos, L. Amigo, and I. Recio
Angiotensin-Converting Enzyme Inhibitory Activity of Peptides Derived from Caprine Kefir
J Dairy Sci, October 1, 2005; 88(10): 3480 - 3487.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
S. Mizuno, S. Nishimura, K. Matsuura, T. Gotou, and N. Yamamoto
Release of Short and Proline-Rich Antihypertensive Peptides from Casein Hydrolysate with an Aspergillus oryzae Protease
J Dairy Sci, October 1, 2004; 87(10): 3183 - 3188.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
M. Murakami, H. Tonouchi, R. Takahashi, H. Kitazawa, Y. Kawai, H. Negishi, and T. Saito
Structural Analysis of a New Anti-Hypertensive Peptide ({beta}-Lactosin B) Isolated from a Commercial Whey Product
J Dairy Sci, July 1, 2004; 87(7): 1967 - 1974.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
R. J. FitzGerald, B. A. Murray, and D. J. Walsh
Hypotensive Peptides from Milk Proteins
J. Nutr., April 1, 2004; 134(4): 980S - 988S.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
G. S. Lv, G. C. Huo, and X. Y. Fu
Expression of Milk-Derived Antihypertensive Peptide in Escherichia coli
J Dairy Sci, June 1, 2003; 86(6): 1927 - 1931.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
V. Vermeirssen, J. Van Camp, K. Decroos, L. Van Wijmelbeke, and W. Verstraete
The Impact of Fermentation and In Vitro Digestion on the Formation of Angiotensin-I-Converting Enzyme Inhibitory Activity from Pea and Whey Protein
J Dairy Sci, February 1, 2003; 86(2): 429 - 438.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Dairy Science Association ®.