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1 Istituto Sperimentale Lattiero Caseario, Via Lombardo 11-26900 Lodi, Italy
2 Dipartimento di Scienza degli Alimenti, Università di Napoli Federico II, Viale Università 100—80055 Portici (Napoli), Italy
3 Dipartimento di Chimica Organica, Parco Area delle Scienze, 43100 Parma, Italy
Pyroglutamic acid is present in many cheese varieties and particularly in high amounts (0.5 g/100 g of cheese) in extensively ripened Italian cheeses (Grana Padano and Parmigiano Reggiano) that are produced with thermophilic lactic acid bacteria as starters. The mechanism of pyroglutamic acid formation in cheese seems to be mostly enzymatic, as demonstrated by the presence of only L-pyroglutamic acid enantiomer. Thermophilic lactobacilli are involved in pyroglutamic acid production, as suggested by the low pyroglutamic acid content found in Bagos, a ripened Italian mountain cheese produced without addition of starter. Because milk pasteurization did not influence the pyroglutamic acid content in the ripened Grana Padano cheese, the formation of pyroglutamic acid mainly depends on the whey starter microflora rather than that of raw milk. Pyroglutamic acid concentration is linearly correlated (R2 = 0.94) with the age of Grana Padano cheese.
Key Words: pyroglutamic acid Grana Padano cheese ripening
Submitted on January 26, 1999
Accepted on November 8, 1999
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