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Journal of Dairy Science Vol. 83 No. 11 2432-2437
© 2000 by American Dairy Science Association ®
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Milk Identification of Different Species: 13C-NMR Spectroscopy of Triacylglycerols from Cows and Buffaloes' Milks

G. Andreotti 1, E. Trivellone 2, R. Lamanna , A. Di Luccia 3, and A. Motta 4

1 Istituto Nazionale di Fisica della Materia, Unità di Ricerca di Salerno, Università degli, Studi di Salerno, I-84081 Baronissi (SA), Italy and Istituto di Chimica delle Molecole di Interesse Biologico and Istituto Nazionale di Chimica dei Sistemi Biologici del CNR, I-80072 Arco Felice (NA), Italy
2 Istituto di Chimica delle Molecole di Interesse Biologico and Istituto Nazionale di Chimica dei Sistemi Biologici del CNR, I-80072 Arco Felice (NA), Italy and Area della Ricerca del CNR di Napoli, I-80072 Arco Felice (NA), Italy
3 Istituto di Scienze dell'Alimentazione del CNR, I-83100 Avellino, Italy
4 Istituto di Chimica delle Molecole di Interesse Biologico and Istituto Nazionale di Chimica dei Sistemi Biologici del CNR, I-80072 Arco Felice (NA), Italy

Triacylglycerols from cows and buffaloes' milk fat were investigated by 13C nuclear magnetic resonance (NMR) spectroscopy. By the addition of pure triacylglycerols standards, we identified the resonances of both milk fats, and the peaks were used for qualitative and quantitative analysis of acyl groups. Multivariate analysis treatment of triacylglycerols distribution and composition parameters enabled us to identify milk. This study shows that NMR can safely be used to quantitate milk fatty acid content, providing unique information for milk identification of different animal species.

Key Words: buffaloes' milk • milk fat • triacylglycerols • 13C-nuclear magnetic resonance

Submitted on March 10, 2000
Accepted on May 13, 2000






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