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Journal of Dairy Science Vol. 82 No. 9 2005-2010
© 1999 by American Dairy Science Association ®
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Screening of Dairy Cows for Ketosis by Use of Infrared Spectroscopy and Multivariate Calibration

Per Waaben Hansen 1

1 Foss Electric A/S, R&D Greenhouse, Slangerupgade 69, DK-3400 Hillerø, Denmark

A fast and inexpensive method of screening ketosis in cows is presented. This method is based on the determination of acetone in bovine milk by using Fourier transform infrared spectroscopy combined with multivariate calibration. Only samples with a naturally increased acetone content could be used in the calibration step; samples containing added acetone did not produce an acceptable calibration equation. On test samples ranging from 0.0 to 2.8 mM acetone, an R2 of 0.81 and an accuracy (root mean square error of prediction) of 0.27 mM were obtained. This accuracy was sufficient to classify the cows into two groups: healthy and possibly ketotic. No false negatives were determined for the present data set.

Key Words: acetone • Fourier transform infrared spectroscopy • ketosis • multivariate calibration

Submitted on November 10, 1998
Accepted on March 26, 1999




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A. P. W. de Roos, H. J. C. M. van den Bijgaart, J. Horlyk, and G. de Jong
Screening for Subclinical Ketosis in Dairy Cattle by Fourier Transform Infrared Spectrometry
J Dairy Sci, April 1, 2007; 90(4): 1761 - 1766.
[Abstract] [Full Text] [PDF]


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