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Journal of Dairy Science Vol. 82 No. 9 1869-1876
© 1999 by American Dairy Science Association ®
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Bifidobacterium bifidum Exhibits a Lipopolysaccharide-Like Mitogenic Activity for Murine B Lymphocytes

E.J. Ko 1, J.S. Goh 2, B.J. Lee 3, S. H. Choi 4, and P.H. Kim 1

1 Department of Microbiology, Kangwon National University, Chunchon 200-701, Korea
2 Department of Animal Products Science, Kangwon National University, Chunchon 200-701, Korea
3 Department of Pharmaceutics, Kangwon National University, Chunchon 200-701, Korea
4 Department of Dairy and Food Sciences, Sangji University, Wonju 220-702, Korea

To determine the role of bifidobacteria in the systemic and mucosal antibody response, we examined the direct modulatory effect of bifidobacteria on the synthesis of antibodies by murine spleen B cells. Whole spleen B cells were cultured with Bifidobacterium bifidum or Clostridium perfringens (Welch's bacilli, negative control), and antibody synthesis was measured by ELISA and enzyme-linked immunospot assay. The B. bifidum, but not C. perfringens, substantially increased total secretion of major immunoglobulin (Ig) isotypes and the number of IgA-secreting cells. In addition, B. bifidum increased proliferation of spleen cells by threefold, and C. perfringens had little to diminishing effect on the cells. These results indicate that B. bifidum increased Ig synthesis through its mitogenic influence on B cells. Further, B. bifidum induced spleen Bcells to be reactive to transforming growth factor-beta1 and interleukin-5 and resulted in increased surface IgA expression (ap threefold) and total IgA production (>20-fold) but not increased production of IgM and IgG2a isotypes.

Together, these studies indicate that B. bifidum can act as a lipopolysaccharide-like polyclonal activator for B cells. Furthermore, that bifidobacteria enable B cells to respond to transforming growth factor-ß1 and interleukin- 5 for the IgA production has important implications for the primary defense against pathogens in the gastrointestinal tract.

Key Words: Bifidobacterium bifidum • B lymphocyte • immunoglobulin • transforming growth factor-ßbeta;1

Submitted on January 5, 1999
Accepted on April 26, 1999




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