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Journal of Dairy Science Vol. 82 No. 3 481-485
© 1999 by American Dairy Science Association ®
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A Novel Method for the Detection of Protease and the Development of Extracellular Protease in Early Growth Stages of Lactobacillus delbrueckii ssp. bulgaricus

Yasushi Kawai 1, Kiyoshi Tadokoro 1, Ryotaro Konomi 1, Kazumi Itoh 1, Tadao Saito 1, Haruki Kitazawa 1, and Takatoshi Itoh 1

1 Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan

A novel method of rapid detection using microplate wells with casein and calcium ions was developed for measuring protease activity. This new method is based on the theory that the hydrolysis by the proteases reduces the turbidity of the solution without coagulation. The three Lactobacillus delbrueckii ssp. bulgaricus strains that were tested secreted a protease with activity that reached its maximum 4 to 8 h after incubation (log phase) and then decreased rapidly. The new method allows the protease activity to be detected and measured more rapidly, easily, and economically than do other methods with fluorescent reagents or radioisotopes, and the new method is especially useful for analyzing large numbers of test samples (e.g., chromatographically fractionated eluates).

Key Words: extracellular protease • Lactobacillus delbrueckii ssp. bulgaricus • lactic acid bacteria • microplate

Submitted on May 12, 1998
Accepted on November 16, 1998




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J. Y. Shin, W. M. Jeon, G.-B. Kim, and B. H. Lee
Purification and Characterization of Intracellular Proteinase from Lactobacillus casei ssp. casei LLG
J Dairy Sci, December 1, 2004; 87(12): 4097 - 4103.
[Abstract] [Full Text] [PDF]


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