Modulation of Fat-Soluble Vitamin Concentrations and Blood Mononuclear Leukocyte Populations in Milk Replacer-Fed Calves by Dietary Vitamin A and ß-Carotene

¹ Metabolic Diseases and Immunology Research Unit, USDA-ARS, National Animal Disease Center, 2300 Dayton Avenue, Ames, IA 50010-0070

Dairy calves (n = 18), separated from dams at birth, were fed 1 L of pooled-colostrum. For the remaining 7 wk of the study, they were fed one of three diets consisting of either a custom-formulated milk replacer without vitamin A (controls), or supplemented with retinyl palmitate (equivalent to 32,000 IU of vitamin A/d) or with ß-carotene (equivalent to 20,000 IU of vitamin A/d). Plasma retinol, ß-carotene, and RRR--tocopherol concentrations were lowest at birth, and increased substantially from birth to 1 wk postpartum in all groups, a probable consequence of ingestion of colostrum. From 1 to 7 wk of age, retinol concentrations were greatest in retinyl palmitate-supplemented calves, intermediate in ß-carotene-supplemented calves and lowest in control calves. At 2, 3, 5, 6, and 7 wk, RRR--tocopherol concentrations were lower in retinyl palmitate-supplemented calves than in control calves. A negative correlation between plasma retinol and vitamin E concentrations existed from wk 2 to 7, suggesting vitamin A influences the absorption and distribution of RRR--tocopherol. Supplemental retinyl palmitate, but not ß-carotene, was associated with a reduction in the percentage of blood mononuclear leukocytes expressing CD2, CD4, and CD8 T cell antigens and interleukin-2 receptors. By wk 7, leukocyte populations from retinyl palmitate-supplemented calves were more similar to those from adult cattle than those from control calves, suggesting that supplemental vitamin A, as retinyl palmitate, affects the maturation of the neonatal immune system. Differences in the composition of blood mononuclear leukocyte populations may represent changes in immune competency.

Key Words: vitamin A status • vitamin E • neonatal calf • immune function

Submitted on May 3, 1999
Accepted on August 19, 1999

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