JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 82 No. 12 2574-2581
© 1999 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bouchard, L.
Right arrow Articles by Lacasse, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bouchard, L.
Right arrow Articles by Lacasse, P.

Nitric Oxide Production During Endotoxin-Induced Mastitis in the Cow

L. Bouchard 1, S. Blais 1, C. Desrosiers 1, X. Zhao 2, and P. Lacasse 1

1 Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, P.O. Box 90, 2000 Route 108 East, Lennoxville (Quebec) Canada J1M 1Z3
2 Department of Animal Science, McGill University, 21,111 Lakeshore Rd, Ste-Anne de Bellevue (Quebec) Canada H9X 3V9

Nitric oxide production was measured during endotoxin-induced mastitis. One hour after morning milking, the right hind quarters of 15 cows were infused with saline containing Escherichia coli endotoxin. Left hind control quarters were infused with saline only. At varying intervals before and after infusion, diagnostic markers of mastitis were recorded and nitric oxide production was evaluated by measuring nitrite plus nitrate levels in milk. In endotoxin-infused quarters, a significant increase in nitrite plus nitrate concentrations was observed 3 h postinfusion; concentrations decreased to preinfusion levels within 48 h. This change indicates that significant amounts of nitric oxide are released during endotoxin-induced mastitis. At 3 different time points, somatic cells were harvested from milk samples, plated, and maintained in culture for 24 h. The concentration of nitrite plus nitrate in medium from cells harvested 12 h postinfusion was increased, suggesting that nitric oxide is released, at least in part, by milk somatic cells. In a second set of experiments, we evaluated nitric oxide production when animals were infused with endotoxin and aminoguanidine, a specific inhibitor of the inducible form of nitric oxide synthase. In cows treated with aminoguanidine, the increase in nitrite plus nitrate observed after endotoxin infusion was prevented. These results suggest that nitric oxide production during endotoxin-induced mastitis resulted from the activity of the inducible form of nitric oxide synthase. They also support a possible involvement for nitric oxide in the inflammatory reaction observed during mastitis.

Key Words: mastitis • nitric oxide • inducible nitric oxide synthase

Submitted on March 23, 1999
Accepted on July 23, 1999




This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
K. Lauzon, X. Zhao, and P. Lacasse
Deferoxamine reduces tissue damage during endotoxin-induced mastitis in dairy cows.
J Dairy Sci, October 1, 2006; 89(10): 3846 - 3857.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
J. Mehrzad, C. Desrosiers, K. Lauzon, G. Robitaille, X. Zhao, and P. Lacasse
Proteases Involved in Mammary Tissue Damage During Endotoxin-Induced Mastitis in Dairy Cows
J Dairy Sci, January 1, 2005; 88(1): 211 - 222.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Dairy Science Association ®.