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Journal of Dairy Science Vol. 82 No. 12 2543-2549
© 1999 by American Dairy Science Association ®
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Isolation of Adipophilin and Butyrophilin from Bovine Milk and Characterization of a cDNA Encoding Adipophilin

R. L. Nielsen 1, M. H. Andersen 1, P. Mabhout 1, L. Berglund 1, T. E. Petersen 1, and J. T. Rasmussen 1

1 Protein Chemistry Laboratory, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Aarhus, Denmark

The milk fat globule membrane-associated proteins adipophilin (alias adipocyte differentiation-related protein) and butyrophilin were purified from bovine milk by reverse-phase chromatography. The nucleotide sequence of bovine adipophilin was obtained via peptide mapping and sequencing of a mammary gland cDNA clone, which comprises 1841 nucleotides and has an open reading frame of 450 amino acids. By peptide mapping, 19% of the amino acid sequence was confirmed. The obtained amino acid sequence has 87 and 80% identical residues with human and mouse adipophilin, respectively. Alignment with the proteins perilipin and TIP47 revealed two highly conserved segments, which may assemble into amphipathic alpha-helices.

Key Words: adipophilin • adipocyte differentiation-related protein • butyrophilin

Submitted on April 28, 1999
Accepted on August 9, 1999




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