JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 81 No. 8 2151-2158
© 1998 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, J.
Right arrow Articles by Smith, K. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, J.
Right arrow Articles by Smith, K. L.

Immunization of Cows with Ferric Enterobactin Receptor from Coliform Bacteria

J. Lin 1, J. S. Hogan 1, M. Aslam 1, and K. L. Smith 1

1 Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691

The serum and milk immunoglobulin (Ig) G responses of lactating dairy cows were determined following immunization with ferric enterobactin receptor FepA. Escherichia coli 471 was cultured in iron-depleted medium, and outer membrane proteins were extracted by 2% N-lauroylsarcosine sodium salt and 2% Triton X-100. The FepA was isolated from the outer membrane proteins by ion-exchange chromatography. Twenty cows were assigned to four treatment groups of 5 cows blocked by breed and days in milk. Treatment groups were vaccinated with 100 µg of FepA, 500 µg of FepA, Escherichia coli J5 bacterin, or sterile phosphate-buffered saline. Primary immunization was at approximately 200 d in milk, and booster immunizations were given 14 and 28 d later. Serum and whey IgG titers to FepA in cows vaccinated with FepA were significantly higher than those from cows vaccinated with either E. coli J5 bacterin or phosphate-buffered saline. Serum and whey IgG titers to FepA were elevated by 14 d in cows vaccinated with FepA. Significant differences were not observed between doses of FepA. The degree of cross-reactivity of purified IgG from cows vaccinated with FepA to E. coli and Klebsiella pneumoniae isolates was significantly higher than that to a control isolate that lacked FepA production. Immunization with FepA elicited an immunological response in serum and milk.

Key Words: ferric enterobactin receptor • mastitis caused by coliform bacteria • vaccine

Submitted on October 14, 1997
Accepted on April 16, 1998




This article has been cited by other articles:


Home page
 J ANIM SCIHome page
M. A. Gorocica-Buenfil, F. L. Fluharty, and S. C. Loerch
Effect of vitamin A restriction on carcass characteristics and immune status of beef steers
J Anim Sci, July 1, 2008; 86(7): 1609 - 1616.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
S. L. Wolf, J. S. Hogan, and K. L. Smith
Iron Uptake by Escherichia coli Cultured with Antibodies from Cows Immunized with High-Affinity Ferric Receptors
J Dairy Sci, July 1, 2004; 87(7): 2103 - 2107.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
K. Takemura, J. S. Hogan, and K. L. Smith
Growth Responses of Escherichia coli to Immunoglobulin G from Cows Immunized with Ferric Citrate Receptor, FecA
J Dairy Sci, February 1, 2004; 87(2): 316 - 320.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
J. Lin, J. S. Hogan, and K. L. Smith
Antigenic Homology of the Inducible Ferric Citrate Receptor (FecA) of Coliform Bacteria Isolated from Herds with Naturally Occurring Bovine Intramammary Infections
Clin. Vaccine Immunol., November 1, 1999; 6(6): 966 - 969.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Dairy Science Association ®.