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Journal of Dairy Science Vol. 81 No. 2 403-410
© 1998 by American Dairy Science Association ®
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Antibiotics Commonly Used to Treat Mastitis and Respiratory Burst of Bovine Polymorphonuclear Leukocytes

D. Hoeben 1, C. Burvenich 1, and R. Heyneman 1

1 Department of Physiology, Biochemistry, and Biometrics, Faculty of Veterinary Medicine, University of Gent, B-9820 Merelbeke, Belgium

The in vitro effects of six doses (2 x 10–3 to 2 x 10–8 M) of antimicrobial drugs that are frequently used in udder infusions on the capacity of bovine blood polymorphonuclear neutrophilic leukocytes to generate reactive oxygen species were studied by the measurement of luminol-dependent chemiluminescence after stimulation with phorbol 12-myristate 13-acetate. All drugs, except cloxacillin, significantly decreased chemiluminescence at the highest dose. Doxycyline induced the most severe inhibition, followed by neomycin and dihydrostreptomycin. The effect of ampicillin was due to the scavenging of reactive oxygen species and interactions with luminol. The inhibition observed with oleandomycin, neomycin, lincomycin, and dihydrostreptomycin was not due to direct effects on the production of oxidative metabolites but rather to interference with other components involved in the production of light, such as interference with the interaction between luminol and the myeloperoxidase-H2O2-halide system. The deleterious effects of doxycycline can be explained by several factors: decreased production of superoxide, yellow color, the scavenging of reactive oxygen species, and Ca2+ chelating effect. In conclusion, the results of this study show that antibiotics may affect neutrophil function at concentrations that are reached in the mammary gland after local and repeated administration.

Key Words: antibiotics • respiratory burst • polymorphonuclear neutrophilic leukocytes

Submitted on July 9, 1997
Accepted on September 16, 1997




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A. G. Zwald, P. L. Ruegg, J. B. Kaneene, L. D. Warnick, S. J. Wells, C. Fossler, and L. W. Halbert
Management Practices and Reported Antimicrobial Usage on Conventional and Organic Dairy Farms
J Dairy Sci, January 1, 2004; 87(1): 191 - 201.
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Copyright © 1998 by the American Dairy Science Association ®.