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Journal of Dairy Science Vol. 80 No. 9 1955-1958
© 1997 by American Dairy Science Association ®
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Influence of Bile on ß-Galactosidase Activity and Cell Viability of Lactobacillus reuteri when Subjected to Freeze-Drying

G. F. de Valdez 1, G. Martos 2, M. P. Taranto 2, G. L. Lorca 2, G. Oliver 2, and A. P. de Ruiz Holgado 2

1 Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000-S.M. Tucumán, Argentina and Cátedra de Microbiología Superior, Facultad Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina
2 Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000-S.M. Tucumán, Argentina

The effect of bile on ß-galactosidase activity and cell viability was investigated using two strains of Lactobacillus reuteri that were subjected to freeze-drying. In the presence of 0.15% oxgall, ß-galactosidase activity of the whole cells was significantly increased. After lyophilization, the cultures that had been treated with oxgall showed a low survival rate without changes in ß-galactosidase activity. The poor resistance of the cells to damage from freeze-drying might be related to the presence of membranous structures containing simple folds and buds of the cell membrane, as was observed by transmission electron microscopy.

Key Words: lactic acid bacteria • bile • ßbeta;-galactosidase • freeze-drying

Submitted on July 22, 1996
Accepted on February 24, 1997






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Copyright © 1997 by the American Dairy Science Association ®.