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1 Department of Animal Sciences, Ohio Agricultural Research, Development Center, The Ohio State University, Wooster 44691
Sixty-six cows and heifers (Holsteins and Jerseys) were assigned to one of three treatments at 60 d before anticipated calving. Treatment 1 consisted of 100 IU/d of supplemental vitamin E during the dry period and 100 IU/d during the first 30 d of lactation. Treatment 2 was 1000 IU/d of vitamin E during the dry period and 500 IU/d during lactation. Treatment 3 was 1000 IU/d of vitamin E during the first 46 d of the dry period, 4000 IU/d during the last 14 d of the dry period, and 2000 IU/d during lactation. Plasma concentrations of a-tocopherol decreased at calving for cows fed dietary treatments with low or intermediate concentrations of vitamin E, but not for cows fed the high vitamin E treatment. High dietary vitamin E increased concentrations of a-tocopherol in blood neutrophils at parturition, but no difference was found for the other two treatments. The percentage of quarters with new infections at calving was not different (32.0%) between cows receiving treatments that contained low and intermediate concentrations of vitamin E but was reduced (11.8%) in cows receiving the high vitamin E treatment. Clinical mastitis affected 25.0, 16.7, and 2.6% of quarters during the first 7 d of lactation for cows receiving the low, intermediate, and high vitamin E treatments, respectively. Cows with plasma concentrations of 
-tocopherol <3.0 µg/ml at calving were 9.4 times more likely to have clinical mastitis during the first 7 d of lactation than were cows with plasma concentrations of -tocopherol >3.0 µg/ml.
Key Words: vitamin E • selenium • mastitis • dairy cows
Submitted on April 15, 1996
Accepted on January 10, 1997
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