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Journal of Dairy Science Vol. 80 No. 2 422-426
© 1997 by American Dairy Science Association ®
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Effect of Freezing on Somatic Cell Count of Quarter Milk Samples as Determined by a Fossomatic Electronic Cell Counter

H. W. Barkema 1, J. Van Der Schans 1, Y. H. Schukken 2, A.L.W. De Gee 1, T.J.G.M. Lam 2, and G. Benedictus 1

1 Animal Health Service, PO Box 361, 9200 AJ Drachten, The Netherlands
2 Department of Herd Health and Reproduction, Utrecht University, PO Box 80151, 3508 TD Utrecht, The Netherlands

The objective of this study was to determine the effect of freezing and the duration of freezing on somatic cell count (SCC) in milk samples from quarters with no signs of clinical mastitis. Effect was determined by a fluoro-opto-electronic cell counter. Samples were frozen in a commercial freezer at approximately –20°C. The milk samples were thawed at 1, 2, 3, 4, 7, 14, or 28 d. Another batch of samples was frozen 1 d, thawed, frozen again for 1 d, and then thawed. The SCC was determined on the fresh samples and after any one of the freezing periods. On average, the SCC were lower after all freezing periods than they were before freezing. However, the difference in SCC before and after freezing was small and relatively constant on the natural logarithm scale. The decrease in SCC was larger with longer freezing periods. When SCC thresholds of 200,000, 250,000, and 500,000 cells/ml were used to predict infection, freezing appeared to have little impact on the sensitivity and specificity of diagnostic parameters. Therefore, SCC, as determined by a fluoro-opto-electronic cell counter, determined for frozen quarter milk samples can be used in mastitis control and research programs.

Key Words: somatic cell count • milk • freezing

Submitted on December 11, 1995
Accepted on June 10, 1996




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