| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Chemistry, The University of Auckland, PB 92019 Auckland, New Zealand
2 New Zealand Rennet Company Ltd., PO Box 122, Eltham, New Zealand
Commercial extracts from oro-pharyngeal tissues of goats and kids have been used as the source of pregastric lipase and have been processed to yield partially purified samples of the primary pregastric lipase. The activity of these lipases against tributyrylglycerol has been determined over a range of pH and temperatures. Optimum pH conditions for pregastric lipase ranged from pH 5.6 to 6.5 for goats and from pH 5.5 to 6.2 for kids, respectively; the optimum temperature ranged from 43 to 60°C. Optima for kid lipase extended slightly below pH 5.5 and higher than 60°C; which were the limits of the test conditions. The enzymes were also used as catalysts for the hydrolysis of monoacid triglycerides (C4:0 to C12:0)at40°C and pH 6.5; activity was maximum against tributyrylglycerol (C4:0) . Values for the Michaelis-Menten constant, increased as carbon chain length of the carboxylic moiety on the triglycerides increased, but values were identical for pregastric lipases of both goats and kids. Anhydrous milk fat was hydrolyzed by the commercial extracts of pregastric lipases of goats and kids, and the resulting profiles for free fatty acids were very similar to one another and to the corresponding profile for a commercial sample of Parmesan cheese. There appear to be no significant differences in activity between the enzyme preparations from goats and kids.
Key Words: caprine pregastric lipase • milk fat • tributyrylglycerol • monoacid triglycerides
Submitted on July 26, 1996
Accepted on April 15, 1997
This article has been cited by other articles:
|
|
 |
|
  N. R. Zwald, K. A. Weigel, Y. M. Chang, R. D. Welper, and J. S. Clay Genetic Selection for Health Traits Using Producer-Recorded Data. I. Incidence Rates, Heritability Estimates, and Sire Breeding Values J Dairy Sci, December 1, 2004; 87(12): 4287 - 4294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. R. Zwald, K. A. Weigel, Y. M. Chang, R. D. Welper, and J. S. Clay Genetic Selection for Health Traits Using Producer-Recorded Data. II. Genetic Correlations, Disease Probabilities, and Relationships with Existing Traits J Dairy Sci, December 1, 2004; 87(12): 4295 - 4302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Calvo and J. Fontecha Purification and Characterization of a Pregastric Esterase From a Hygienized Kid Rennet Paste J Dairy Sci, May 1, 2004; 87(5): 1132 - 1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Fenster, S. A. Rankin, and J. L. Steele Accumulation of Short n-Chain Ethyl Esters by Esterases of Lactic Acid Bacteria Under Conditions Simulating Ripening Parmesan Cheese J Dairy Sci, September 1, 2003; 86(9): 2818 - 2825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. O'Connor and R. D. Manuel Calf Pregastric Lipase Catalyzed Hydrolysis of 4-Nitrophenylalkanoates and Anhydrous Milk Fat: Effect of pH, Temperature and Acyl Carbon Chain Length Journal of Bioactive and Compatible Polymers, September 1, 2000; 15(5): 406 - 424. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
