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Journal of Dairy Science Vol. 80 No. 1 94-100
© 1997 by American Dairy Science Association ®
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Effect of Prolactin on In Vitro Expression of the Bovine Mammary Immunoglobulin G1 Receptor

G. M. Barrington 1, T. E. Besser 1, C. C. Gay 1, W. C. Davis 1, J. J. Reeves 1, and T. B. McFadden 2

1 Washington State University, Pullman 99164
2 Ruakura Agricultural Centre, Hamilton, New Zealand

Explants of mammary tissue from cows in late pregnancy were incubated for 72 h in serum-free, hormonally defined media to investigate the regulation of the bovine mammary IgG1 receptor. Treatments included incubation in basal medium alone, basal medium plus estradiol-17ß, basal medium plus prolactin, or basal medium plus estradiol-17ß and prolactin. alpha-Lactalbumin production was measured by radioimmunoassay in culture supernatants collected at 24, 48, and 72 h. Explants were examined immunohistochemically for expression of the IgG1 receptor at 24, 48, and 72 h. alpha-Lactalbumin concentrations increased, and IgG1 receptor expression decreased, by 72 h with explants cultured in the medium containing prolactin. Results suggest that, in addition to its positive lactogenic effect, prolactin decreases expression of the bovine mammary IgG1 receptor.

Key Words: prolactin • bovine • mammary • immunoglobulin G1

Submitted on August 4, 1995
Accepted on May 20, 1996






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Copyright © 1997 by the American Dairy Science Association ®.