Effects of Saccharomyces cerevisiae and Aspergillus oryzae Cultures on Ruminal Fermentation in Dairy Cows

¹ Department of Animal Science, University of Minnesota, St. Paul 55108

Four lactating Holstein cows, fitted with ruminal and duodenal cannulas, were used in a 4 x 4 Latin square design to examine the effects of supplemental yeast (Saccharomyces cerevisiae) and fungal (Aspergillus oryzae) cultures on ruminal fermentation, microbial populations, and nutrient supply to the small intestine. Cows were fed a basal diet comprising 32.5% corn silage, 17.5% alfalfa hay, 35.3% corn grain, 12.7% soybean meal, and 2% vitamin and mineral mixture on a DM basis. Treatment were arranged in a 2 x 2 factorial as follows: 1) basal diet, 2) basal diet plus 57 g/d of yeast culture, 3) basal diet plus 3 g/d of fungal culture, and 4) basal diet plus 57 g/d of yeast culture and 3 g/d of fungal culture. Ruminal pH, ammonia N concentration, and total VFA concentration were similar among treatments. Molar percentages of ruminal isoacids were lower for cows fed a mixture of yeast and fungal culture than for cows fed yeast or fungal culture alone. Yeast culture increased ruminal OM and CP digestion and decreased OM and N flow to the duodenum. Fiber digestion in the rumen was similar among treatments. Fungal culture stimulated proteolytic and cellulolytic bacterial counts. Proteolytic bacterial counts were also stimulated by yeast culture. Results from this experiment demonstrated that yeast and fungal cultures could influence ruminal fermentation and microbial populations.

Key Words: direct-fed microbials • Saccharomyces cerevisiae • Aspergillus oryzae • ruminal fermentation

Submitted on January 10, 1995
Accepted on October 27, 1995

This article has been cited by other articles:

				L. Liou, H. Sheng, W. Ferens, C. Schneider, A. N. Hristov, I. Yoon, and C. J. Hovde Reduced Carriage of Escherichia coli O157:H7 in Cattle Fed Yeast Culture Supplement Professional Animal Scientist, October 1, 2009; 25(5): 553 - 558. [Abstract] [PDF]

				G. J. Lascano, G. I. Zanton, F. X. Suarez-Mena, and A. J. Heinrichs Effect of limit feeding high- and low-concentrate diets with Saccharomyces cerevisiae on digestibility and on dairy heifer growth and first-lactation performance J Dairy Sci, October 1, 2009; 92(10): 5100 - 5110. [Abstract] [Full Text] [PDF]

				D. Moya, S. Calsamiglia, A. Ferret, M. Blanch, J. I. Fandino, L. Castillejos, and I. Yoon Effects of dietary changes and yeast culture (Saccharomyces cerevisiae) on rumen microbial fermentation of Holstein heifers J Anim Sci, September 1, 2009; 87(9): 2874 - 2881. [Abstract] [Full Text] [PDF]

				R. A. Longuski, Y. Ying, and M. S. Allen Yeast culture supplementation prevented milk fat depression by a short-term dietary challenge with fermentable starch J Dairy Sci, January 1, 2009; 92(1): 160 - 167. [Abstract] [Full Text] [PDF]

				S. P. Oliver, D. A. Patel, T. R. Callaway, and M. E. Torrence ASAS Centennial Paper: Developments and future outlook for preharvest food safety J Anim Sci, January 1, 2009; 87(1): 419 - 437. [Abstract] [Full Text] [PDF]

				D. G. V. Emmanuel, A. Jafari, K. A. Beauchemin, J. A. Z. Leedle, and B. N. Ametaj Feeding live cultures of Enterococcus faecium and Saccharomyces cerevisiae induces an inflammatory response in feedlot steers J Anim Sci, January 1, 2007; 85(1): 233 - 239. [Abstract] [Full Text] [PDF]

				I. R. Ipharraguerre and J. H. Clark Impacts of the Source and Amount of Crude Protein on the Intestinal Supply of Nitrogen Fractions and Performance of Dairy Cows J Dairy Sci, May 1, 2005; 88(e_suppl_1): E22 - E37. [Abstract] [Full Text] [PDF]

				B. Mwenya, B. Santoso, C. Sar, B. Pen, R. Morikawa, K. Takaura, K. Umetsu, K. Kimura, and J. Takahashi Effects of Yeast Culture and Galacto-Oligosaccharides on Ruminal Fermentation in Holstein Cows J Dairy Sci, April 1, 2005; 88(4): 1404 - 1412. [Abstract] [Full Text] [PDF]

				K. E. Lesmeister, A. J. Heinrichs, and M. T. Gabler Effects of Supplemental Yeast (Saccharomyces cerevisiae) Culture on Rumen Development, Growth Characteristics, and Blood Parameters in Neonatal Dairy Calves J Dairy Sci, June 1, 2004; 87(6): 1832 - 1839. [Abstract] [Full Text] [PDF]

				K. A. Beauchemin, W. Z. Yang, D. P. Morgavi, G. R. Ghorbani, W. Kautz, and J. A. Z. Leedle Effects of bacterial direct-fed microbials and yeast on site and extent of digestion, blood chemistry, and subclinical ruminal acidosis in feedlot cattle J Anim Sci, June 1, 2003; 81(6): 1628 - 1640. [Abstract] [Full Text] [PDF]

				T. Miller-Webster, W. H. Hoover, M. Holt, and J. E. Nocek Influence of Yeast Culture on Ruminal Microbial Metabolism in Continuous Culture J Dairy Sci, August 1, 2002; 85(8): 2009 - 2014. [Abstract] [Full Text] [PDF]