JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 79 No. 12 2237-2246
© 1996 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burton, J. L.
Right arrow Articles by Mallard, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burton, J. L.
Right arrow Articles by Mallard, B. A.

Effects of Supplemental Chromium on Production of Cytokines by Mitogen-Stimulated Bovine Peripheral Blood Mononuclear Cells

J. L. Burton 1, B. J. Nonnecke 2, P. L. Dubeski 3, T. H. Elsasser 4, and B. A. Mallard 5

1 Department of Animal Science, Michigan State University, East Lansing 48824
2 USDA, ARS, National Animal Disease Center, Ames, IA 50010-0070
3 Agriculture and Agri-Food Canada, LaCombe Research Center, LaCombe, AB, Canada T4L 1W1
4 USDA, ARS, Growth Biology Laboratory, Beltsville, MD 20507
5 Veterinary Microbiology and Immunology, University of Guelph, Guelph, ON, Canada N1G 2W1

This study determined whether supplementing the diets of dairy cows during the peripartum period with organic trivalent Cr influenced the capacity of their peripheral blood mononuclear cells to produce activation cytokines in response to stimulation with mitogens in vitro. Nine cows were fed 0.5 ppm of Cr/d per cow from 6 wk prepartum to 16 wk postpartum; 10 other periparturient cows served as unsupplemented controls. Mononuclear leukocytes, enriched from peripheral blood during wk 0, 2, 4, and 6 of lactation, were cultured with or without the T-lymphocyte mitogen, concanavalin A. Culture supernatants, harvested at 24, 48, or 72 h, were assayed for interleukin-2, interferon-gamma, and tumor necrosis factor-alpha. The cytokines were barely detectable in the supernatants from the unstimulated cultures, but supernatants from mitogen-stimulated cultures contained higher concentrations of each cytokine. For cows fed Cr, concentrations of all three cytokines in the culture supernatants of the mitogen-stimulated mononuclear cells decreased significantly relative to values for unsupplemented cows, particularly around peak lactation for the 24- and 48-h cultures. Theses results extended our previous observations and supported the hypothesis that organic Cr is immunomodulatory in high producing cows.

Key Words: chromium • cytokines • mononuclear cells • immunomodulation

Submitted on April 20, 1995
Accepted on April 19, 1996




This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
M. A. Bryan, M. T. Socha, and D. J. Tomlinson
Supplementing Intensively Grazed Late-Gestation and Early-Lactation Dairy Cattle with Chromium
J Dairy Sci, December 1, 2004; 87(12): 4269 - 4277.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Dairy Science Association ®.