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Journal of Dairy Science Vol. 79 No. 11 1988-1991
© 1996 by American Dairy Science Association ®
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Detection of Ergot (Claviceps purpurea) in a Dairy Feed Component by Gas Chromatography and Mass Spectrometry

P. G. Mantle 1

1 Biochemistry Department, Imperial College of Science, Technology and Medicine, London, United Kingdom SW7 2AY

A dairy feed that contained barley malt screenings caused hyperthermia in dairy cattle. The feed was suspected of containing ergot and was subsequently analyzed to determine the ricinoleate component, a special and prominent feature of oil-rich ergot tissue. Triglyceride oil was extracted by organic solvents from the dairy feed, and the oil was saponified to release fatty acids. Ricinoleate, as a methyl ester, was selectively resolved from other fatty acids by silica gel chromatography and was analyzed by capillary gas chromatography coupled with chemical ionization mass spectrometry, which demonstrated the presence of the methyl ricinoleate molecule and proved that the feed contained ergot. The methodology may be refined to monitor for ergot in powdered dairy feed more routinely.

Key Words: ergot analysis • dairy feed • gas chromatography • mass spectrometry

Submitted on May 2, 1995
Accepted on April 9, 1996






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