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Journal of Dairy Science Vol. 79 No. 1 76-82
© 1996 by American Dairy Science Association ®
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Mastitis-Causing Escherichia coli: Serum Sensitivity and Susceptibility to Selected Antibacterials in Milk

W. Fang 1 and S. Pyörälä 2

1 Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Helsinki, Fin-00014, Helsinki, Finland
2 Department of Clinical Sciences, Faculty of Veterinary Medicine University of Helsinki, Fin-00014, Helsinki, Finland

A total of 169 Escherichia coli strains were isolated from cows with cases of clinical mastitis. ß-Glucuronidase production, serum sensitivity, and susceptibility to selected antibacterials were analyzed using the fluorometric ß-glucuronidase assay. About 89% (150 to 169) of the isolates tested positive for ß-glucuronidase. Of these isolates producing ß-glucuronidase, 102 (68%) were resistant or moderately resistant bovine serum. The antibacterial susceptibility of 96 isolates was tested in broth and milk. There was a significant shift from lower fluorometric minimum inhibitory concentration for tetracycline, sulfadoxin-trimethoprim, enrofloxacin, and gentamicin in broth to higher fluorometric minimum inhibitory concentration in milk. Serum sensitivity and susceptibility to tested antibacterials in broth or in milk were not related. Gentamicin and sulfadoxin-trimethoprim seemed to be more potent in mastitic milk than in normal milk, suggesting a possible synergistic effect between these exogenous antibacterials and the indigenous antibacterial agents in mastitic milk.

Key Words: Escherichia coli • ßbeta;-glucuronidase • antibacterial susceptibility • mastitis

Submitted on April 24, 1995
Accepted on September 11, 1995




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