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Journal of Dairy Science Vol. 79 No. 1 33-43
© 1996 by American Dairy Science Association ®
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Modulation of Proliferation, Second Messenger Levels, and Morphotype Expression of the Rat Intestinal Epithelial Cell Line IEC-6 by Fermented Milk

Karine Thoreux 1, Francoise Senegas-Balas 2, Francoise Bernard-Perrone 2, Sylvie Giannarelli 2, Gerard Denariaz 3, Christine Bouley 3, and Daniel Balas 2

1 Laboratoire d'Histologie, Faculté de Médecine de Nice, Avenue Valombrose, 06107 Nice, Cedex 2, France
2 Laboratoire d'Histologie, Faculté de Médecine de Nice, Avenue Valombrose, 06107 Nice, Cedex 2, France
3 Groupe Danone et Centre International de Recherche Daniel Carasso 15 avenue Galilée, Le Plessis Robinson 92350, Cedex, France

Trophic effects of milk fermented with Lactobacillus helveticus, Lactobacillus paracasei ssp. paracasei, Bifidobacterium sp., or the combination of Lactobacillus bulgaricus and Streptococcus thermophilus (yogurt) were studied on the IEC-6 intestinal epithelial cell line. Incorporation of [methyl-3H]thymidine, mitochondrial dehydrogenase activities, cyclic AMP production, ad differentiation of levels of the IEC-6 strain were evaluated between the 15th and 30th passage in culture. All fermented and unfermented milks enhanced trophic responses of IEC-6 cells in a dose-dependent manner. Compared with the corresponding milks, supernatant fractions were more effective in stimulating mitochondrial dehydrogenase response. Fermented milk supernatants were also more effective than the corresponding unfermented fractions. Increases in DNA synthesis and cyclic AMP confirmed the activation observed with mitochondrial dehydrogenase. Yogurt induced the more trophic response with an increased number of the more differentiated cell morphotype. Fermentation with L. casei also demonstrated an important trophic adaptation of IEC-6 cells. Milk processing by lactic acid bacteria enhanced trophic and proliferation responses of intestinal epithelial cell line IEC-6. These results suggested that IEC-6 cells could represent an accurate and easy in vitro model for testing the trophic quality of various nutrients and for an optimization of physioloical digestive functions.

Key Words: lactic acid bacteria • fermented milk • IEC-6 cells • cell proliferation

Submitted on October 19, 1994
Accepted on April 28, 1995




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Copyright © 1996 by the American Dairy Science Association ®.