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Journal of Dairy Science Vol. 79 No. 1 16-19
© 1996 by American Dairy Science Association ®
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Alkaline Phosphatase Activity in Penicillium roqueforti and in Blue-Veined Cheeses

I. Rosenthal 1, S. Bernstein 1, and B. Rosen 1

1 Department of Food Science, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel

The Penicillium roqueforti mold exhibits alkaline phosphatase activity. Therefore, blue mold-ripened cheeses made from properly pasteurized milk test positive for phosphatase. Because microbial phosphatases are considered to be more resistant to heat than is milk phosphatase, a statutory control test recommends the repasteurization of cheese at 66°C fir 30 min to inactivate selectively the native milk enzyme. However, because of thermal lability of Penicillium roqueforti phosphatase, this control test leads to confusion of the fungal enzyme with native milk alkaline phosphatase and does not confirm whether the milk used to make cheese has been pasteurized.

Key Words: Penicillium roqueforti • alkaline phosphatase • testing • pasteurization

Submitted on October 5, 1994
Accepted on July 11, 1995




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