Isolation and Characterization of Temperate and Virulent Bacteriophages of Lactobacillus plantarum

¹ Area de Microbiología, Departamento de Biología Funcional, Universidad de Oviedo, 33006-Oviedo, Spain

Several bacteriophages that are able to infect Lactobacillus plantarum have been isolated by induction of lysogenic strains with mitomycin C and by enrichment of samples from different origins. Two closely related phages (LP1-A and LP1-B), isolated from corn silage, and phage LP2, isolated from a homemade cheese whey, were characterized. Some features of L. plantarum phage fri were also studied that have not been previously published. Virions of the LP1 and LP2 groups displayed a typical B1 morphology (Siphoviridae); heads were isometric, and tails were long and noncontractile. The genetic material of the virions was a single molecule of double-stranded DNA without cohesive ends. The genome lengths were 80 kbp (LP1 group), 47 kbp (LP2), and 133 kbp (fri). Host range was limited to some strains of L. plantarum. Temperature of propagation affected the appearance and aspect of the plaques of lysis. Phage adsorption was quite efficient, reaching >90% in most cases and often >99%, and was not affected by the temperature. One-step growth experiments showed that, in some cases, temperature strongly influenced the phage development. The temperate nature of the phages could only be established for phage LP2, and no lysogens were obtained for members of the LP1 group.

Key Words: Lactobacillus plantarum • bacteriophages • isolation • characterization

Submitted on February 22, 1994
Accepted on October 17, 1994

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