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Journal of Dairy Science Vol. 78 No. 12 2745-2752
© 1995 by American Dairy Science Association ®
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Antigenic Crossreactivity and Lipopolysaccharide Neutralization Properties of Bovine Immunoglobulin G

G. M. Tomita 1, D. A. Todhunter 1, J. S. Hogan 1, and K. L. Smith 1

1 Department of Animal Science, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691

We investigated a possible mechanism by which immunization against core and lipid A determinants of lipopolysaccharide reduced clinical cases of mastitis and symptoms commonly associated with heterologous Gram-negative IMI. The IgG fraction of sera from cows immunized with either Escherichia coli J5 bacterin, E. coli J5 lipopolysaccharide conjugate vaccine, or unimmunized controls was purified by precipitation with caprylic acid and ammonium sulfate. The degree of IgG crossreactivity with Gramnegative bacteria that were isolated from clinical quarters was greater than that with Gram-positive isolates of Staphylococcus aureus. The highest magnitude of crossreactivity was against smooth strain E. coli isolates, followed by heterologous species of Enterobacter, Serratia, and Klebsiella isolates. Serum IgG from cows immunized with conjugate was highly crossreactive to E. coli J5, E. coli O111:B4, Serratia marcescens, Klebsiella pneumoniae, and Salmonella typhimurium lipopolysaccharides. The magnitude of antibody crossreactivity with lipopolysaccharides coincided with the ability of IgG to suppress the mitogenic effect of lipopolysaccharides on bovine lymphocytes.

Key Words: mastitis • lipopolysaccharide • immunizationi • immunoglobulin G

Submitted on November 30, 1994
Accepted on August 14, 1995




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Copyright © 1995 by the American Dairy Science Association ®.