Application of Capillary Electrophoresis in the Identification of Phenotypes Containing the ß-Lactoglobulin C Variant

¹ Food Science Section, New Zealand Dairy Research Institute, Private Bag 11029, Palrnerston North, New Zealand
² Food Science Section, New Zealand Dairy Research Institute, Private Bag 11029, Palrnerston North, New Zealand

A method for the separation of the ß-LG variants A, B, and C was developed using free zone capillary electrophoresis. A separation buffer consisting of 50 mM 2-(N-morpholino)ethane sulfonic acid (pH 8.0) and polyoxyethylene (20)-sorbitan monolaurate was used for the separation. Milk samples from 424 Jersey cows were phenotyped using this method, and the gene frequencies for ß-LG A, B, and C were .41, .53, and .06, respectively. These frequencies were different from those found in studies of Jersey populations in other countries, where the ß-LG B allele had a significantly higher frequency, and the ß-LG A allele a significantly lower frequency, than those of this present study. The frequency of the ß-LG C allele was intermediate to those of other studies. The concentration of ß-LG in the milk was different among the ß-LG phenotypes and was, from greatest to least: AA, AB, AC, BB, and BC.

Key Words: ßbeta;-lactoglobulin • phenotypes • milk protein variants • capillary electrophoresis

Submitted on October 12, 1994
Accepted on August 15, 1995

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