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Journal of Dairy Science Vol. 78 No. 11 2382-2387
© 1995 by American Dairy Science Association ®
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Distribution of Annexins I, II, and IV in Bovine Mammary Gland

Norio Katoh 1, Takafumi Suzuki 2, Akira Yuasa 2, and Toru Miyamoto 3

1 Laboratory of Biochemistry, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305, Japan
2 Department of Veterinary Biochemistry, Rakuno Gakuen University, Ebetsu 069, Japan
3 Hokkaido Branch Laboratory, National Institute of Animal Health, 4 Hitsujigaoka, Sapporo 062, Japan

Annexins belong to a family of proteins that are characterized by their ability to bind phospholipids in a Ca2+-dependent manner that is thought to be involved in a variety of biological processes. The present study determined the localization of annexins in subcellular fractions, nuclei in particular, of COW mammary gland by immunoblot analysis using monoclonal antibodies to annexins I, II, IV, and VI. The analysis revealed that annexins I, 11, and IV were present in cytosol, but VI was not. Annexins I and IV were found in the nuclear fraction, but annexin II was only faintly present. Annexin VI was also undetectable in this fraction. Cytosolic annexin I had a molecular mass of 36 kDa. The 36-kDa annexin I was also found in the nuclear fraction. A 38-kDa annexin I was additionally detected in nuclei. The cytosolic and nuclear 36-kDa annexin I and the nuclear 38-kDa annexin I showed different isoelectric points, as revealed by two-dimensional PAGE. Annexin IV from cytosolic and nuclear fractions had similar molecular masses and isoelectric points.

Key Words: annexins • mammary gland

Submitted on January 17, 1995
Accepted on July 10, 1995




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