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Journal of Dairy Science Vol. 78 No. 11 2375-2381
© 1995 by American Dairy Science Association ®
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Influence of Mastitis on D-Amino Acid Content of Milk

János Csapó 1, Zsuzsanna Csapó-Kiss 1, József Stefler 1, Truman G. Martin 2, and Sándor Némethy 3

1 Pannon Agricultural University Faculty of Animal Science, H-7401 Kaposvár, Dénesmajor 2, Hungary
2 Department of Animal Science, Purdue University, West Lafayette, IN
3 Department of Marine Geology, University of Göteborg, Göteborg, Sweden

The California Mastitis Test was used as an indicator of mastitis in this study. Five cows were chosen for each of the five test scores (from 0 = healthy to 4 = severe mastitis). Milk samples were analyzed for free AA and free D-AA. The contents of free AA, free D-AA, and the ratio of free D-AA to free AA increased significantly as the California Mastitis Test score increased. The free D-AA content of foremilk (first milk jets) from healthy cows (test score = 0) was approximately five times that in samples drawn later from the same udders. Contents of free AA and free D-AA were highly associated with test score and udder inflammation. Very low concentrations of free D-AA are normal for raw milk. Higher concentrations of free D-AA could be attributed to inclusion of foremilk and milk from cows having subclinical mastitis in the bulk tank milk.

Key Words: free amino acids • D-amino acids • mastitis • milk

Submitted on December 28, 1994
Accepted on May 26, 1995




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S. Rubio-Barroso, M. J. Santos-Delgado, C. Martin-Olivar, and L. M. Polo-Diez
Indirect Chiral HPLC Determination and Fluorimetric Detection of D-Amino Acids in Milk and Oyster Samples
J Dairy Sci, January 1, 2006; 89(1): 82 - 89.
[Abstract] [Full Text] [PDF]


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Copyright © 1995 by the American Dairy Science Association ®.