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Journal of Dairy Science Vol. 78 No. 11 2352-2357
© 1995 by American Dairy Science Association ®
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Preparation of Apolactoferrin with a Very Low Iron Saturation

M. Feng 1, L. Van Der Does 1, and A. Bantjes 1

1 Department of Chemical Technology, Biomaterials Section, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands

Iron(III) removal from lactoferrin by an iron(III)-chelating resin with immobilized 3-hydroxy-2-methyl-4(1H)-pyridinone ligands was studied at physiological pH in the presence of citrate. The resin had a marked effect on the extent of iron removal. By using the iron(III)-chelating resin, removal of iron from lactoferrin was nearly complete in <24 h. Apolactoferrin with 4% iron saturation could be prepared conveniently from 100% or from 18% iron-saturated lactoferrin under mild conditions without affecting the iron-binding capacity of the protein. The iron saturation of the obtained apolactoferrin was much lower than that of the apolactoferrin prepared by reported methods.

Key Words: lactoferrin • apolactoferrin • iron removal • chelating resin

Submitted on February 6, 1995
Accepted on June 20, 1995






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