JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 78 No. 1 91-102
© 1995 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iqbal, N.
Right arrow Articles by Hunter, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iqbal, N.
Right arrow Articles by Hunter, A. G.

Effect of Various Capacitation Systems on Bovine Sperm Motion Characteristics, Acrosome Integrity, and Induction of Hyperactivation

N. Iqbal 1 and A. G. Hunter 1

1 Department of Animal Sciences, University of Minnesota, St. Paul 55108

Sperm from the same ejaculate were capacitated in TEST-yolk, calcium-free Tyrode's, modified Tyrode's containing follicular fluid or heparin, and analyzed at 0, 4, 8, and 12 h for motility characteristics and acrosomal integrity. Percentage of motility, progressive motility, progressive velocity, path velocity, curvilinear velocity, linearity, and amplitude of lateral head displacement differed with media and decreased with time. Calcium-free Tyrode's ranked first in maintaining most motility parameters. However, TEST-yolk sperm ranked first for lateral head displacement but last for linearity at each time. Acrosome-reacted sperm increased over time but were not different between TEST-yolk, calcium-free Tyrode's, and heparin at 4 h. The TEST-yolk sperm at 4 h had about twice the hyperactivation (62.3%) of the other sperm capacitation media. Capacitation of bovine sperm was not associated with a specific change in any measured motility parameter. Differences among the efficacies of capacitation systems were unrelated to parameters of sperm motion, hyperactivation, or acrosomal integrity.

Key Words: capacitation • sperm • motion parameters • computer-assisted motility analysis

Submitted on March 28, 1994
Accepted on August 26, 1994




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
F. Martinez-Pastor, V. Garcia-Macias, M. Alvarez, P. Herraez, L. Anel, and P. de Paz
Sperm Subpopulations in Iberian Red Deer Epididymal Sperm and Their Changes Through the Cryopreservation Process
Biol Reprod, February 1, 2005; 72(2): 316 - 327.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. M. Henricks, A. J. Kouba, B. R. Lackey, W. R. Boone, and S. L. Gray
Identification of Insulin-Like Growth Factor I in Bovine Seminal Plasma and Its Receptor on Spermatozoa: Influence on Sperm Motility
Biol Reprod, August 1, 1998; 59(2): 330 - 337.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by the American Dairy Science Association ®.