Galactopoietic Effects of a (1-30)NH₂ Analog of Growth Hormone-Releasing Factor in Dairy Cows

¹ Department of Animal Science, Animal Reproduction Laboratory, Michigan State University, East Lansing 48824, and The Upjohn Company, 237921-25-4, Performance Enhancement Research, Kalamazoo, MI 49001

Two experiments were conducted to determine the galactopoietic effects of an analog of growth hormone-releasing factor. Specific replacements were made in the AA sequence of growth hormone-releasing factor to produce a growth hormone-releasing factor analog with enhanced stability in vivo. In Experiment 1, 24 Holstein cows were infused i.v. with 0, .33, 1, or 3 mg/d of growth hormone-releasing factor analog for 20 d. Infusion of the analog increased mean serum concentrations of somatotropin, IGF-I, and NEFA. The analog also increased milk yield as much as 44% relative to that of controls. In Experiment 2, 24 Holstein cows were infused i.v. with 0, .11, .33, or 1 mg/d of analog for 60 d. Infusion of analog increased serum concentrations of somatotropin, IGF-I, and NEFA in a dose-dependent manner. At 1 mg/d, the analog increased SCM yield 28% throughout the infusion period relative to the 0 mg/d controls. In summary, a growth hormone-releasing factor analog increased milk yield and serum concentrations of somatotropin and IGF-I. Furthermore, this galactopoietic activity was mediated through the somatotropic axis.

Key Words: analog • growth hormonereleasing factor • milk yield • somatotropin

Submitted on December 9, 1993
Accepted on April 18, 1994