Categorical Data Analysis of the Effect on Bull Fertility, of Butylated Hydroxytoluene Addition to Semen, Extenders Prior to Freezing

¹ The Pennsylvania State University, University Park 16802, and Eastern Artificial Insemination Cooperative, Ithaca, NY 14851
² The Pennsylvania State University, University Park 16802, and Eastern Artificial lnsemination Cooperative, Ithaca, NY 14851

Butylated hydroxytoluene is an anti-oxidant that has antiviral properties and sustains sperm viability during freezing and thawing. A field trial involving 11 bulls and 19,000 AI was conducted to determine whether addition of .5 mM butylated hydroxytoluene to whole milk extender during seminal processing affected bull fertility as estimated by non-return rates generated by cows bred to the bulls. Effects of bull, batch of semen nested within bull, treatment, and month of AI were studied. Nonreturn rates were recorded for each month for every bull, batch of semen (ejaculates pooled on a given day), and treatment combination. Because some bulls had <6 batches of semen, the original experimental design was reduced to two smaller designs. Categorical data analysis with maximum likelihood estimation was used for analysis of nonreturn rates. The results from three models were used to interpret the data. The nonreturn rates were approximately 73.9% for the butylated hydroxytoluene treatment and 74.1 % for the control. In all models, bull effect was significant, but batch, month of AI, and treatment had no effect on bull fertility. Addition of .5 mM butylated hydroxytoluene to whole milk extender during semen processing did not affect bull nonreturn rates.

Key Words: cryopreservation • statistics • antioxidant • sperm

Submitted on August 19, 1993
Accepted on March 14, 1994

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