JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 77 No. 8 2214-2220
© 1994 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, C. S.
Right arrow Articles by Keller, W. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, C. S.
Right arrow Articles by Keller, W. L.

Modulation of Expression of fos and Ha-ras Oncogenes and Ornithine Decarboxylase Activity in Mammary Gland and Liver of Young Female Rats by the Absence of Dietary Lipotropes

C. S. Park 1, C. B. Choi 1, M. G. Baik 1, and W. L. Keller 1

1 Laboratory of Growth and Lactation, Department of Animal Sciences, North Dakota State University, Fargo 58105

There is evidence that diets deficient in lipotropes [methionine, choline, pteroylmonoglutamic acid (folic acid), and cyanocobalamin (vitamin B12)] induce and enhance hepatocarcinogenesis. This research examined the extent to which dietary lipotropes modify cellular oncogene expression and ornithine decarboxylase activity in mammary gland and liver of rats. Eighteen female Sprague-Dawley rats (8 wk old) were fed 3 wk on one of three diets: 1) a control synthetic diet; 2) a methyl-deficient diet lacking choline, methionine, pteroylmonoglutamic acid, and cyanocobalamin; or 3) a diet supplemented with twice the amount of each lipotrope as in the control synthetic diet. The group fed the methyl-deficient diet gained less body weight than groups fed the control or methyl-supplemented diet. The group fed the methyl-deficient diet had approximately 5– and 11-fold greater fos transcription in mammary gland and liver, respectively, than did the control group. The expression of the Ha-ras gene in mammary gland and liver of the group fed the methyl-deficient diet was increased by 4- and 6-fold compared with that of the control. Ornithine decarboxylase activity, considered to be a developmental marker, was higher in liver and mammary gland of the group fed the methyl-deficient diet than in either the group fed control synthetic diet or the group fed the methyl-supplemented diet. The methyl-deficient diet may have caused activation of the transcription factor fos and thus the activation of the transcription regulatory complex, AP-1. In turn, AP-1 may regulate genes, such as ornithine decarboxylase, which are responsible for cell proliferation and differentiation.

Key Words: oncogenes • ornithine decarboxylase • rats

Submitted on November 22, 1993
Accepted on March 11, 1994




This article has been cited by other articles:


Home page
 J. Nutr.Home page
J. Santos-Guzman, T. Arnhold, H. Nau, C. Wagner, S. H. Fahr, G. E. Mao, M. A. Caudill, J. C. Wang, S. M. Henning, M. E. Swendseid, et al.
Antagonism of Hypervitaminosis A-Induced Anterior Neural Tube Closure Defects with a Methyl-Donor Deficiency in Murine Whole-Embryo Culture
J. Nutr., November 1, 2003; 133(11): 3561 - 3570.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
K. Wu, K. J. Helzlsouer, G. W. Comstock, S. C. Hoffman, M. R. Nadeau, and J. Selhub
A Prospective Study on Folate, B12, and Pyridoxal 5'-Phosphate (B6) and Breast Cancer
Cancer Epidemiol. Biomarkers Prev., March 1, 1999; 8(3): 209 - 217.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Dairy Science Association ®.