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Journal of Dairy Science Vol. 77 No. 6 1761-1767
© 1994 by American Dairy Science Association ®
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Evaluation of the Hydrometer for Testing Immunoglobulin G1 Concentrations in Holstein Colostrum

Lori C. Pritchett 1, Clive C. Gay 1, Dale D. Hancock 1, and Thomas E. Besser 1

1 College of Veterinary Medicine, Washington State University, Pullman 99164-6610

Hydrometer measurement in globulin and IgG1 concentration measured by the radial immunodiffusion technique were compared for 915 samples of first milking colostrum from Holstein cows. Least squares analysis of the relationship between hydrometer measurement and IgG1 concentration was improved by log transformation of IgG1 concentration and resulted in a significant linear relationship between hydrometer measurement and log10 IgG1 concentration; r2 = .469.

At 50 mg of globulin/ml of colostrum, the recommended hydrometer cutoff point for colostrum selection, the sensitivity of the hydrometer as a test of IgG1 concentration in Holstein colostrum was 26%, and the negative predictive value was 67%. The negative predictive value and sensitivity of the hydrometer as a test of IgG1 in Holstein colostrum was improved, and the cost of misclassification of colostrum was minimized, when the cutoff point for colostrum selection was increased above the recommended 50 mg/ml.

Key Words: colostrum • colostrometer • immunoglobulin G1 • specific gravity

Submitted on October 7, 1993
Accepted on January 28, 1994




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A. Arguello, N. Castro, and J. Capote
Short Communication: Evaluation of a Color Method for Testing Immunoglobulin G Concentration in Goat Colostrum
J Dairy Sci, May 1, 2005; 88(5): 1752 - 1754.
[Abstract] [Full Text] [PDF]


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