JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 77 No. 5 1251-1258
© 1994 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sanchez, M. S.
Right arrow Articles by Yancey, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sanchez, M. S.
Right arrow Articles by Yancey, R. J., Jr.

Effect of Tumor Necrosis Factor-alpha, Interleukin-1ß, and Antibiotics on the Killing of Intracellular Staphylococcus aureus

M. S. Sanchez 1, C. W. Ford 1, and R. J. Yancey Jr. 1

1 The Upjohn Company, Kalamazoo, MI 49001

A bovine blood polymorphonuclear neutrophil system was used to determine the ability of cytokines, alone or in combination with various antibiotics, to enhance the intracellular killing of Staphylococcus aureus sequestered within these phagocytes. Pretreatment of blood polymorphonuclear neutrophils with human recombinant tumor necrosis factor-alpha, bovine macrophage supernatant tumor necrosis factor-alpha, or human recombinant interleukin-1ß enhanced the killing of S. aureus by the phagocytes compared with that of untreated polymorphonuclear neutrophils. This enhancement was dose-dependent and required 30 min of contact between the cytokine and the blood polymorphonuclear neutrophils. However, mammary gland polymorphonuclear neutrophils, macrophages, and blood monocytes showed no enhanced killing activity following pretreatment with tumor necrosis factor. Pretreatment of polymorphonuclear neutrophils with tumor necrosis factor and subsequent exposure to various antibiotics after infection with S. aureus enhanced intracellular bacterial killing by certain antibiotics, including paldimycin, rifampin, and ciprofloxacin (antibiotics that normally kill intracellular S. aureus). However, no enhancement occurred with antibiotics that do not normally kill intracellular S. aureus. Cytokine therapy may be a useful adjunct to antibiotic therapy in the treatment of bovine staphylococcal infection and mastitis.

Key Words: polymorphonuclear neutrophil • antibiotics • Staphylococcus aureus • tumor necrosis factor-alpha

Submitted on August 2, 1993
Accepted on December 20, 1993




This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y. Ishida, T. Hayashi, T. Goto, A. Kimura, S. Akimoto, N. Mukaida, and T. Kondo
Essential Involvement of CX3CR1-Mediated Signals in the Bactericidal Host Defense during Septic Peritonitis
J. Immunol., September 15, 2008; 181(6): 4208 - 4218.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
H. Sarikaya, G. Schlamberger, H. H. D. Meyer, and R. M. Bruckmaier
Leukocyte populations and mRNA expression of inflammatory factors in quarter milk fractions at different somatic cell score levels in dairy cows.
J Dairy Sci, July 1, 2006; 89(7): 2479 - 2486.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
M. W. Pfaffl, S. L. Wittmann, H. H. D. Meyer, and R. M. Bruckmaier
Gene Expression of Immunologically Important Factors in Blood Cells, Milk Cells, and Mammary Tissue of Cows
J Dairy Sci, February 1, 2003; 86(2): 538 - 545.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Dairy Science Association ®.