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Journal of Dairy Science Vol. 77 No. 11 3324-3330
© 1994 by American Dairy Science Association ®
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Effects of In Vivo Administration of 1,25-Dihydroxyvitamin D3 on In Vitro Proliferation of Bovine Lymphocytes

F. G. Hustmyer 1, D. C. Beitz 1, J. P. Goff 2, B. J. Nonnecke 2, R. L. Horst 2, and T. A. Reinhardt 2

1 Department of Animal Science, Molecular, Cellular, and Developmental Biology Program, Iowa State University, Ames 50011
2 USDA, Agricultural Research Service, National Animal Disease Center, Metabolic Diseases and Immunology Research Unit, Ames, IA 50010-0070

Continuous infusion of 1,25-dihydroxyvitamin D3 in the bovine elevated 1,25-dihydroxyvitamin D3 in plasma. Plasma 1,25-dihydroxyvitamin D3 remained elevated until infusions were discontinued. Increased 1,25-dihydroxyvitamin D3 was associated with hypercalcemia (11 to 12 mg/dl) that continued for several days postinfusion. Elevated 1,25-dihydroxyvitamin D3 also was associated with a significant 70% enhancement of lymphocyte proliferation in cells treated with concanavalin A. Cessation of infusion of 1,25-dihydroxyvitamin D3 resulted in the return of 1,25-dihydroxyvitamin D3 in plasma and lymphocyte proliferative response to preinfusion amounts. Proliferation in response to pokeweed mitogen was significantly inhibited on d 3 of infusions of 1,25-dihydroxyvitamin D3. Administration of parathyroid hormone for 4 d resulted in a sustained hypercalcemia (ap 13 mg/dl) and a transient 1-d increase in 1,25-dihydroxyvitamin D3 in plasma. Parathyroid hormone treatment generally did not affect the response of bovine lymphocytes to mitogen. In vivo elevation of 1,25-dihydroxyvitamin D3 is either moderately immunostimulating or exerts no effect on mitogen-stimulated proliferation of lymphocytes. These results contrast with the in vitro results suggesting that 1,25-dihydroxyvitamin D3 is only immunosuppressive.

Key Words: immune function • vitamin D • parathyroid hormone

Submitted on December 16, 1993
Accepted on May 23, 1994




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M. R. Waldron, B. J. Nonnecke, T. Nishida, R. L. Horst, and T. R. Overton
Effect of Lipopolysaccharide Infusion on Serum Macromineral and Vitamin D Concentrations in Dairy Cows
J Dairy Sci, November 1, 2003; 86(11): 3440 - 3446.
[Abstract] [Full Text] [PDF]


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Copyright © 1994 by the American Dairy Science Association ®.