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Journal of Dairy Science Vol. 77 No. 10 2949-2958
© 1994 by American Dairy Science Association ®
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Production of Various Forms of Plasminogen Activator and Plasminogen Activator Inhibitor by Cultured Mammary Epithelial Cells

C. E. Heegard 1, J. H. White 1, B. Zavizion 1, J. D. Turner 1, and I. Politis 1

1 Department of Animal and Food Sciences, University of Vermont, Burlington 05405

We examined amounts and types of plasminogen activator and plasminogen activator inhibitor produced by cultured bovine mammary epithelial cells. The MAC-T and two other mammary epithelial cell lines, MACT-UV1 and MACT-UV2 derived from the parental MAC-T cells by subcloning, were used as model systems. Cells were cultured in a medium free of serum and protein. Data showed that MACT-UV2 cells produced 6.2 and 17.2% more plasminogen activator than MACT-UV1 and parental MAC-T cells, respectively. Addition of amiloride, a specific urokinaseplasminogen activator inhibitor, dramatically decreased the activity in the culture medium of parental and subclonal lines, indicating that urokinase-plasminogen activator was present. Zymography revealed the presence of urokinaseplasminogen activator with an approximate molecular mass of 50,000 kDa in the culture medium of parental MAC-T cells. The culture medium of the subclonal lines contained urokinaseplasminogen activator and tissueplasminogen activator with approximate molecular masses of 50,000 and 75,000 kDa, respectively. Complexes of both types of plasminogen activators with plasminogen activator-inhibitor-1 were detected in the culture medium of subclonal lines.

Key Words: plasminogen activator • plasminogen activator-inhibitor-1 • epithelial cells • involution

Submitted on February 4, 1994
Accepted on May 9, 1994






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Copyright © 1994 by the American Dairy Science Association ®.