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Journal of Dairy Science Vol. 76 No. 6 1740-1743
© 1993 by American Dairy Science Association ®
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Use of Modified Rambach Agar to Differentiate Streptococcus uberis from Other Mastitis Streptococci

Jeffrey L. Watts 1, Sarah A. Salmon 1, and Robert J. Yancey Jr. 1

1 Animal Health Therapeutics Research, The Upjohn Company, Kalamazoo, MI 49001

The medium of Rambach was modified to permit differentiation of mastitis streptococci. A total of 377 streptococci isolated from bovine IMI was used in the study. Of the 159 strains identified as Streptococcus uberis, 151 strains (94.9%) were ß-galactosidase-positive and yielded blue colonies on modified Rambach agar. In comparison, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus saccharolyticus, or Enterococcus faecalis strains were negative for propylene glycol utilization (red colonies) or ß-galactosidase production; all strains yielded colorless colonies on modified Rambach agar. However, 5 of 35 (14.3%) Streptococcus equinus strains were also positive for ß-galactosidase. Results indicate that modified Rambach agar is a convenient medium for the differentiation of the Strep. uberis from the other mastitis streptococci. Furthermore, modified Rambach agar could be easily incorporated as a screening medium for Strep. uberis in mastitis bacteriology laboratories.

Key Words: streptococci • Streptococcus uberis • Rambach agar • differential

Submitted on December 23, 1992
Accepted on February 11, 1993






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Copyright © 1993 by the American Dairy Science Association ®.