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Journal of Dairy Science Vol. 76 No. 5 1403-1411
© 1993 by American Dairy Science Association ®
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Identity and Paternity Testing of Cattle: Application of a Deoxyribonucleic Acid Profiling Protocol

Gerjan J. M. Trommelen 1, Nanke H. G. Den Daas 2, Jan Vijg 3, and André G. Uitterlinden 3

1 INGENY B. V. PO Box 685 2300 AR Leiden, The Netherlands
2 Holland Genetics PO Box 454 6800 AL Amhem, The Netherlands
3 INGENY B.V. PO Box 685 2300 AR Leiden, The Netherlands

We have applied DNA profiling for identity and parentage studies of cattle using a standardized procedure based on synthetic micro- and minisatellite multilocus core probes in Southern blot hybridization assays. This protocol is useful for paternity analysis of cattle and for real case work (e.g., identity and paternity disputes).

Key Words: paternity testing • deoxyribonucleic acid fingerprinting • variable number of tandem repeat loci • core probes

Submitted on February 3, 1992
Accepted on January 4, 1993







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