JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 76 No. 5 1260-1267
© 1993 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Politis, I.
Right arrow Articles by Gorewit, R. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Politis, I.
Right arrow Articles by Gorewit, R. C.

Enzymatic Assay for the Combined Determination of Plasmin Plus Plasminogen in Milk: Revisited

I. Politis 1, B. Zavizion 1, D. M. Barbano 1, and R. C. Gorewit 1

1 Northeast Dairy Foods Research Center, Department of Animal Science, Cornell University Ithaca, NY 14853

The ability of ß-lactoglobulin variants A and B, alpha-lactalbumin, and BSA to inhibit plasmin plus plasminogen activity was examined. Data showed that ß-lactoglobulin A at concentrations of .2 and 1 mg/ml inhibited plasmin plus plasminogen activity by 18 and 54%. ß-Lactoglobulin B had no effect on plasmin plus plasminogen activity. At concentrations of .2 and 1 mg/ml, BSA inhibited plasmin plus plasminogen activity by 25 and 63%. alpha-Lactalbumin at concentrations of .2 and 1 mg/ml caused 1.9 and 20% inhibition of plasmin plus plasminogen activity. These data, collectively, suggest that existing methodology for measuring plasmin activity in milk serum underestimates real plasmin activity in milk. Underestimation is more pronounced in samples with high whey protein content (late lactation milk and milk obtained from mastitic quarters). To avoid this problem, we have modified the existing methodology. Our modification allows plasmin determination without interference from whey proteins and other plasmin inhibitors that are present in the serum fraction of bovine milk.

Key Words: plasmin • plasminogen • whey proteins

Submitted on September 11, 1992
Accepted on December 17, 1992




This article has been cited by other articles:


Home page
 J DAIRY SCIHome page
L. Wang, K. D. Hayes, and L. J. Mauer
Fluorescent Labeling Study of Plasminogen Concentration and Location in Simulated Bovine Milk Systems
J Dairy Sci, January 1, 2006; 89(1): 58 - 70.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
J. Mehrzad, C. Desrosiers, K. Lauzon, G. Robitaille, X. Zhao, and P. Lacasse
Proteases Involved in Mammary Tissue Damage During Endotoxin-Induced Mastitis in Dairy Cows
J Dairy Sci, January 1, 2005; 88(1): 211 - 222.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
K. M. Rippel, S. S. Nielsen, and K. D. Hayes
Effects of Native and Denatured Whey Proteins on Plasminogen Activator Activity
J Dairy Sci, August 1, 2004; 87(8): 2344 - 2350.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
M. R. Garcia-Risco, I. Recio, E. Molina, and R. Lopez-Fandino
Plasmin Activity in Pressurized Milk
J Dairy Sci, March 1, 2003; 86(3): 728 - 734.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Dairy Science Association ®.